HBsAg阳性孕妇新生儿T细胞亚群测定

目的:通过检测HBsAg阳性孕妇分娩的新生儿T细胞亚群,探讨HBV宫内感染与T细胞亚群之间的关系。方法:以太原市第三人民医院妇产科68例HBsAg阳性孕妇分娩的新生儿作为研究对象,应用酶联免疫吸附法检测孕妇和新生儿出生24 h内外周血乙肝病毒标志物,利用实时荧光定量PCR检测母亲和新生儿外周血HBV-DNA含量,使用流式细胞术检测新生儿股静脉血中的CD3+、CD4+、CD8+T细胞亚群,并分析HBV宫内感染与T细胞亚群表达的关系。结果:宫内感染组新生儿股静脉血T细胞亚群CD3+、CD4+、CD8+与未宫内感染组相比升高,CD4+/CD8+与未宫内感染组新生儿相比降低,宫内感染和非宫内感染组新生儿股静脉血T细胞亚群含量比较,差异无统计学意义(P>0.05)。结论:尚未观察到HBV宫内感染新生儿T细胞亚群比例失调。

Detection of T lymphocytes subgroups of neonates born by HBsAg positive pregnant women

Objective:To explore the relationship between HBV intrauterine infection and T lymphocytes subgroups by detecting T lymphocytes subgroups of neonates born by HBsAg positive pregnant women.Methods:The neonates borne by 68 HBsAg positive pregnant women from department of gynecology and obstetrics in the Third People’s Hospital of Taiyuan City were selected as study objects,ELISA was used to detect HBV markers in peripheral blood samples of pregnant women and neonates within 24 hours after birth,HBV-DNA contents in peripheral blood samples of mothers and neonates were detected by real-time fluorescent quantitative PCR method,flow cytometry was used to detect the subgroups of CD3+,CD4+,CD8+ T lymphocytes in femoral venous blood samples of neonates,the relationship between HBV intrauterine infection and the expressions of T lymphocytes subgroups was analyzed.Results:Compared with non-intrauterine infection group,the subgroups of CD3+,CD4+,CD8+ T lymphocytes in femoral venous blood samples of neonates in intrauterine infection group increased,the ratio of CD4+/CD8+ decreased;there was no statistically significant difference in the contents of T lymphocytes subgroups in femoral venous blood samples of neonates between non-intrauterine infection group and intrauterine infection group(P>0.05).Conclusion:Ratio imbalance of T lymphocytes subgroups in neonates with HBV intrauterine infection is not observed.