卡巴胆碱对肠缺血-再灌注大鼠血浆肿瘤坏死因子-α和白介素-10含量的影响

目的：研究拟胆碱药卡巴胆碱对肠缺血－再灌注大鼠血浆肿瘤坏死因子－α（TNF－α）、白介素－10（IL－10）和皮质醇含量的影响及其意义。方法：成年雄性Wistar大鼠，戊巴比妥钠腹腔麻醉，用动脉夹阻断肠系膜上动脉血流(SMAO），1h后松夹恢复灌流。随机数字表法将大量分为预防、治疗和对照3组，预防组和治疗组分别在夹闭后30min和再灌注后30min肌肉注射卡巴胆碱（0.1mg/kg)，对照组注射等量生理盐水。各组分别于未夹闭前（0h)和夹闭后1h,2.5h和6h测定血浆中TNF－α、IL－10和皮质醇含量。结果：大鼠缺血－再灌注损伤后，血浆TNF－α、IL－10和皮质醇含量均明显升高。肌肉注射卡巴胆碱后，治疗组及预防组血浆TNF－α含量在各时间点均明显低于对照组（P均＜0．01），同时预防组TNF－α含量在1h和6h点低于治疗组（P均＜0．05）；3组动物之间血浆IL－10和皮质醇含量在夹闭后各时间点均无明显差异。结论：卡巴胆碱能抑制肠缺血－再灌注大鼠体内促炎细胞因子的产生，而对抗炎细胞因子的释放影响轻微，有助于减轻缺血－再灌注损伤的全身性炎症反应。

Effects of carbachol on plasma levels of tumor necrosis factor-α and interleukin-10 in rats during gut ischemia-reperfusion

OBJECTIVE: To investigate the effects of carbachol on the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10) and cortisol in plasma of rats during gut ischemia-reperfusion. METHODS: Wistar rats were anaesthetized with soluble pentobarbitone, and subjected to superior mesenteric artery occlusion (SMAO) for 60 minutes, followed by reperfusion for 60 minutes. Animals were divided into three groups, pretreated group (intramuscular injection carbachol at 30 minutes after SMAO, 0.1 mg/kg), treated group (intramuscular injection of carbachol at 30 minutes after reperfusion), and control group (saline injected). The levels of TNF-alpha, IL-10 and cortisol in plasma were determined at 0 hour, 1 hour, 2.5 hours and 6 hours after SMAO. RESULTS: The levels of TNF-alpha, IL-10 and cortisol significantly increased after SMAO (P<0.01 ). The levels of TNF-alpha significantly decreased in pretreated and treated groups than that in control after the intramuscular injection of carbachol (all P<0.01). However, the levels of IL-10 and cortisol did not show significant differences among three groups. It was also found that lower content of TNF-alpha in pretreated group than that in treated group, especially at 1 hour and 6 hours. CONCLUSION: The results suggest that carbachol reduce the proinflammatory cytokine releasing and has a less inhibiting effect on the anti-inflammatory cytokine. It is indicated that carbachol play a potential role in alleviating systemic inflammatory response during splanchnic ischemia-reperfusion injury.