全反式维甲酸对EC9706荷瘤裸鼠抑瘤作用的实验研究

目的：观察全反式维甲酸的体内抗肿瘤作用，探讨其体内抑瘤的作用机制。方法：采用裸鼠EC9706人食管癌细胞实体瘤模型进行体内抑瘤实验研究，应用全反式维甲酸10d后处死裸鼠，观察荷瘤裸鼠的肿瘤生长情况，测定肿瘤生长抑制率；HE染色对肿瘤组织进行细胞形态学观察，TUNEL法检测肿瘤组织中细胞凋亡，免疫组织化学法检测凋亡相关基因bax及bcl-2蛋白的表达情况。结果：全反式维甲酸对裸鼠EC9706人食管癌细胞实体瘤生长具有明显的抑制作用，抑瘤率达60％以上。组织学观察及TUNEL检测结果显示，肿瘤组织中散在凋亡细胞和凋亡小体。免疫组织化学检测结果显示，凋亡相关基因bax蛋白表达增加，bcl-2蛋白表达下降。结论：全反式维甲酸具有较强的体内抗肿瘤作用，作用机制与诱导肿瘤细胞凋亡有关。

All-trans retinoic acid inhibited the growth of human esophageal cancer EC9706 cells in nude mice

Objective:To investigate the anti-tumor effect of alltrans retinoic acid(ATRA) in vivo and its mechanisms.Methods:Human esophageal cancer EC9706 cells were transplanted into nude mice to establish the solid tumor model.We observed the tumor growth and calculated the tumor growth inhibition rate after administration of all-trans retinoic acid.Ten days after all-trans retinoic acid treatment,morphological changes of tumor tissues were examined by HE staining.Apoptosis was detected by TUNEL assay and the protein expression of apoptosis-associated gene bax and bcl-2 were tested by immunohistochemical method.Results:All-trans retinoic acid significantly inhibited the growth of human esophageal cancer EC9706 cells in nude mice,and the tumor growth inhibition rate was more than 60%.Histological examination and TUNEL staining revealed the scattered apoptotic cells and apoptotic bodies in tumor tissues.The protein expression of apoptosis-associated gene bax was increased and that of bcl-2 was decreased.Conclusion:All-trans retinoic acid has stronger anti-tumor effect in vivo.The mechanism of its anti-tumor effect might be mediated by inducing the apoptosis of tumor cells.