土贝母中1个活性皂苷的分离和鉴定

目的：研究中药土贝母中的生物活性成分。方法：采用稻瘟霉模型生物活性追踪方法,应用多种色谱技术进行分离,根据波谱解析和化学手段鉴定其结构。结果：分离并鉴定了1个具有诱导稻瘟霉菌丝变形活性的双糖链皂苷：3-O-α-L-吡喃阿拉伯糖(1→2)-β-D-吡喃葡萄糖-贝萼皂苷元-28-O-β-D-吡喃木糖(1→3)-α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖酯苷(Ⅰ)。结论：Ⅰ为新天然产物,对K-562和BEL-7402肿瘤细胞显示显著的细胞毒性,但对1%兔红细胞无溶血作用。

Isolation and structural elucidation of a bioactive saponin from tubers of Bolbostemma paniculatum

OBJECTIVE: To study the bioactive constituents from tubers of Bolbostemma paniculatum. METHOD: Compounds were isolated by extraction and partition as well as several-chromatographic techniques guided with Pyricularia oryzae bioassay method. Their structures were determined on the basis of spectral analysis and chemical evidence. RESULT: Bisdesmoside (I) was isolated as active compound causing morphological abnormality of Pyncularia oryzae mycelia and elucidated as 3-0-alpha-L-arabinopyranosyl (1-->2)-beta-D-glucopyranosyl-bayogenin-28-O-beta-D-xylopyranosyl (1-->3)-alpha-L-rhamnopyranosyl (1-->2)-alpha-L-arabinopyranoside. CONCLUSION: I is a new natural product and exhibited significant cytotoxicity against cancer cell lines K-562 and BEL-7402, but no hemolytic activity to rabbit erythrocytes.