Novel multiplex PCR using dual-priming oligonucleotides for detection and discrimination of the Mycobacterium tuberculosis complex and M. bovis BCG |
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| Authors: | Lee Hye Ryun Kim So Yeon Chang Ho Eun Song Sang Hoon Lee Hye Seung Park Kyoung Un Song Junghan Kim Eui-Chong |
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| Affiliation: | Department of Laboratory Medicine, Seoul National University Bundang Hospital, 463-707, Gumi-dong, Seongnam-si, Bundang-gu, Gyeonggi-do, South Korea. |
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| Abstract: | We developed a novel multiplex PCR assay using dual-priming oligonucleotide primers targeting the RD1 gene for simultaneous identification of the Mycobacterium tuberculosis complex and M. bovis bacillus Calmette-Guérin (BCG). This assay would be useful both for detection of the M. tuberculosis complex and for differentiation of M. bovis BCG from pathogenic M. tuberculosis complex species. |
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