Full-depth epidermis tomography using a Mirau-based full-field optical coherence tomography |
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| Authors: | Chien-Chung Tsai Chia-Kai Chang Kuang-Yu Hsu Tuan-Shu Ho Ming-Yi Lin Jeng-Wei Tjiu Sheng-Lung Huang |
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| Institution: | 1.Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan;2.Department of Dermatology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan;3.Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan;4.Email: |
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| Abstract: | With a Gaussian-like broadband light source from high brightness Ce3+:YAG single-clad crystal fiber, a full-field optical coherence tomography using a home-designed Mirau objective realized high quality images of in vivo and excised skin tissues. With a 40 × silicone-oil-immersion Mirau objective, the achieved spatial resolutions in axial and lateral directions were 0.9 and 0.51 μm, respectively. Such a high spatial resolution enables the separation of lamellar structure of the full epidermis in both the cross-sectional and en face planes. The number of layers of stratum corneum and its thickness were quantitatively measured. This label free and non-invasive optical probe could be useful for evaluating the water barrier of skin tissue in clinics. As a preliminary in vivo experiment, the blood vessel in dermis was also observed, and the flowing of the red blood cells and location of the melanocyte were traced.OCIS codes: (060.2380) Fiber optics sources and detectors, (170.4500) Optical coherence tomography, (160.1435) Biomaterials, (170.3880) Medical and biological imaging, (180.3170) Interference microscopy |
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