载脂蛋白E多态性与冠心病患者脂代谢的临床研究

目的研究载脂蛋白E(apoE)多态性与冠心病(CHD)及其脂代谢之间的关系。方法采用等电点聚焦电泳免疫印迹技术测定91例CHD及105例正常对照者的apoE表型，并用酶法测定他们的脂质水平。结果CHD组和对照组apoE表型分布及等位基因频率无显著性差异(P＞0．05)。CHD组不同apoE表型之间血脂水平比较发现，apoE基因多态性与胆固醇(TC)(P＜0．01)、低密度脂蛋白胆固醇(LDL-C)(P＜0．01)、甘油三酯(TG)(P＜0．05)有关，即E4等位基因携带者具有较高的TC和LDL-C水平，而E2等位基因携带者具有较低的TC和LDL-C水平。E2和E4等位基因携带者TG水平均升高。结论apoE基因多态性影响CHD患者的脂代谢，并通过影响脂代谢在CHD中起重要作用，但不是CHD的一个独立危险因素。

Clinical study of the association between apoE polymorphism and lipid metabolism of patients with CHD

Objective:To study the relationship between apoE polymorphism and blood lipid level.Method: apoE phenotype was determined in 91 patients with CHD and 105 normal controls by isoelectric focusing and immunoblotting.The lipids of these subjects were measured with enzymes methods.Result:There were no differences in the apoE phenotype distribution and apoE allele frequencies (P＞0.05) between two groups. Comparasion of values of serum lipid with various apoE phenotype among paitents with CHD revealed that there was correlation between apoE polymorphism and TC (P＜0.01),LDL-C (P＜0.01)and TG(P＜0. 05)。 Patients carrying E4 were associated with increased TC and LDL-C,while those with E2 were assiociated with decreased TC and LDL-C.Patients carrying E2 and E4 were associated with increased TG.Conclusion:These data suggest that apoE polyphorphism affects lipid metabolism of patients with CHD,and play an important role in CHD,but it is not an independent risk factor of CHD.