肺炎链球菌临床分离株耐药性及红霉素耐药相关基因的研究

目的了解本院临床肺炎链球菌感染状况、耐药性以及耐药基因流行情况。方法2004年1月~2005年12月从患者不同标本分离鉴定肺炎链球菌,采用NCCLS标准K-B法对临床常用抗生素进行耐药分析、PCR检测红霉素耐药基因ermB和mefA。结果分离129株肺炎链球菌,其中青霉素敏感率为42.4%,红霉素耐药率为80.6%。104株红霉素耐药肺炎链球菌中,ermB基因检出率为97.1%,mefA基因检出率为41.3%,41株同时含有ermB和mefA基因。结论本院临床分离肺炎链球菌耐药性高,红霉素耐药基因主要为ermB。

Antibiotic resistance and erythromycin resistance related genes investigation of isolated Streptococcus pneumoniae

Objective To investigate drug susceptibility and erythromycin resistance genes of isolated Streptococcus(S.) pneumoniae from Southwest Hospital.Methods S.pneumoniae were collected from clinic wards from January 2004 to December 2005.Antibioltic sensitivity test was done by using K-B method.Genes related to erythromycin resistance such as ermB,mefA were detected by PCR.Results In all 129 strains,the susceptibility rate of penicillin were 42.4%;the resistance rate of erythromycin were 80.6%;the positive rate of ermB and mefA gene were 97.1%,41.3%,respectively in 104 strains erythromycin resistanced S.pneumoniae.Both ermB and mefA gene were found in 41 strains.Conclusion Antibiotic resistance of S.pneumoniae in local region were high.S.pneumoniae at this region was erythromycin resistant by ermB gene.