平阳降压方对自发性高血压大鼠肝阳上亢证模型血压及胰岛素抵抗的作用

目的：观察平阳降压方对自发性高血压大鼠（spontaneous hypertensiverats，SHR）肝阳上亢证模型的血压及胰岛素抵抗（insulin resistance，IR）的影响，并探讨其降压作用及可能机制。方法：24只SHR随机分为4组，每组6只，均给予附子汤连续灌胃4周，复制成肝阳上亢模型。Wistar大鼠6只，作为正常对照组。从第5周开始，4组SHR分别给予平阳降压方低、高剂量煎剂（8、16g／kg），尼群地平混悬液（1．6mg／kg））及蒸馏水连续灌胃30d，每天1次。第1天灌胃后，每2h测量一次各组大鼠尾动脉收缩压，共24h，观察药物的急性降压作用；在灌服药物过程中每3d测量一次各组大鼠尾动脉收缩压，观察其慢性降压作用。并观察治疗前后各组大鼠空腹血糖（fasting plasmaglucose，FPG）、空腹胰岛素（fasting insulin，FINS）、胰岛素敏感指数（insulin sensitivity index，ISI）及抵抗素（resistin）的变化情况。结果：（1）急性降压实验中，平阳降压方低、高剂量煎剂及尼群地平均能有效降低SHR血压（P〈0．05）。三组间24h总的降压效果无统计学差异（P〉0．05），但平阳降压方低、高剂量组24h内血压变化较尼群地平组平稳（P〈0．05）；慢性降压实验中平阳降压方低、高剂量煎剂及尼群地平均能明显降低SHR血压（P〈0．05）。高剂量平阳降压方煎剂降压效果优于低剂量组（P〈0．05）。（2）治疗后平阳降压方低、高剂量组及尼群地平组SHR的FINS及抵抗素较治疗前均明显降低（P〈0．05），ISI均明显增高（P〈0．05），平阳降压方低、高剂量煎剂改善IR的效果优于尼群地平（P〈0．05）。结论：（1）平阳降压方对sHR肝阳上亢证模型有明显的急慢性降压作用。（2）平阳降压方具有改善SHR肝阳上亢证模型IR的作用。

Effects of Ping Yang Jiang Ya Fang on blood pressure and insulin resistance in Gan Yang Shang Kang Syndrome model of spontaneous hypertensive rats

AIM:To observe the effect of Ping Yang Jiang Ya Fang on blood pressure and insulin resistance(IR)in Gan Yang Shang Kang Syndrome model of spontaneous hypertensive rats(SHR),and to explore its hypotensive effect and possible underlying mechanisms.METHODS:Twenty-four SHR were randomly divided into four groups with 6 rats in each group,all of the SHR were drenched with Aconiti Praeparatae Decoction for four weeks to make Gan Yang Shang Kang Syndrome model.Another six healthy Wistar rats were taken as normal control group.From the fifth week,each SHR group was drenched with low or high dose of Ping Yang Jiang Ya Fang Decoction(8 g/kg,16 g/kg),the suspension of nitrendipine(1.6 mg/kg)or distilled water for thirty consective days,once a day.After the first intragastric administration,the tail artery systolic pressure of all rats was measured at every two hours for twenty-four hours in order to observe the acute hypotensive effect of drugs.And it was also measured at every three days during drenching drugs in order to observe its chronic hypotensive effect.The changes of the fasting plasma glucose(FPG),fasting insulin(FINS),insulin sensitivity index(ISI)and resistin of all rats were also determined before and after therapy.RESULTS:(1)In the acute hypotensive experiment,low or high dose of Ping Yang Jiang Ya Fang Decoction and nitrendipine could reduce the tail artery systolic pressure of SHR(P＜0.05),but there was no significant difference in twenty-four hours among them(P＞0.05).The changes of systolic pressure of SHR in the groups with low dose or high dose of Ping Yang Jiang Ya Fang Decoction were smaller than those with nitrendipine during twenty-four hours(P＜0.05).In the chronic hypotensive experiment,they could also decrease the tail artery systolic pressure of SHR(P＜0.05).The hypotensive effect of high dose of Ping Yang Jiang Ya Fang Decoction was better than that of low dose(P＜0.05).(2)The plasma FINS and resistin of SHR in the groups of low or high dose of Ping Yang Jiang Ya Fang Decoction,or nitrendipine were significantly decreased(P＜0.05),while the ISI was significantly increased(P＜0.05)after treatment.And the effect of either low or high dose of Ping Yang Jiang Ya Fang Decoction was better than that of nitrendipine(P＜0.05).CONCLUSION:(1)Ping Yang Jiang Ya Fang has obvious acute and chronic hypotensive effects to Gan Yang Shang Kang Syndrome model of SHR.(2)Ping Yang Jiang Ya Fang can improve the IR of Gan Yang Shang Kang Syndrome model of SHR.