榄香烯体内外抑制小鼠肝癌H_（22）细胞增殖与诱导凋亡作用的研究

目的]研究中药莪术提取物榄香烯制剂（Elemene）对小鼠肝癌H22细胞增殖的抑制及诱导凋亡的作用。方法]体内实验,通过建立H22小鼠肝癌实体瘤模型,观察榄香烯对荷瘤小鼠肿瘤生长的抑制作用,TdT酶介导的原位缺口末端标记法（TUNEL）检测凋亡细胞。体外进行细胞培养,采用MTT法检测榄香烯对细胞增殖的影响,Annexin V-FITC/PI双染色法检测细胞的凋亡。结果]不同实验组对荷瘤小鼠肿瘤生长的抑制作用程度不同,5-氟脲嘧啶组抑制肿瘤生长最明显,榄香烯高剂量组较低剂量组抑瘤效果明显;MTT检测到榄香烯对H22细胞株具有抑制作用,呈剂量和作用时间的依赖性;Annexin V-FITC/PI法检测到早期凋亡细胞;TUNEL法检测到凋亡细胞。结论]榄香烯可有效抑制小鼠肝癌H22细胞的增殖,其抑制癌细胞增殖的途径是通过诱导细胞发生凋亡而完成的。

Antitumor effects of elemene on the proliferation inhibition and apoptosis induction in ascites hepatoma cell line H22

Objective]To investigate the antitumor effects of elemene on the proliferation inhibition and apoptosis induction in ascites hepatoma cell line H22.Methods]Mouse models of the solid tumor of liver cancer were established.The inhibitory rates of solid tumor were observed.The rates of cell viability were observed by MTT.Apoptotic cells were detected by AnnexinV-FITC/PI staining and TUNEL labeling methods.Results]After H22cells were treated with various concentrations of drug,cells growth were significantly...