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A porous 3D cell culture micro device for cell migration study |
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| Authors: | Liang Ma Changchun Zhou Biaoyang Lin Wei Li |
| Affiliation: | (1) Department of Mechanical Engineering, University of Washington, Seattle, WA 98195-2600, USA;(2) Swedish Medical Center, Seattle, WA 98122, USA;(3) Department of Urology, University of Washington, Seattle, WA 98195, USA;(4) Department of Mechanical Engineering, The University of Texas at Austin, Austin, TX 78712, USA; |
| Abstract: | Cell migration under chemoattractant is an important biological step in cancer metastasis that causes the spread of malignant tumor cells. Porous polymeric materials are widely used to mimic the extracellular matrix (ECM) environment for applications such as three dimensional (3D) cell culturing and tissue engineering. In this paper we report a novel 3D cell culture device based on porous polymeric material to study cancer migration. We fabricated a porous channel on a polymeric chip using a selective ultrasonic foaming method. We demonstrate that a chemical concentration gradient could be established through the porous channel due to the slow diffusion process. We show that significant cell migration could be observed through the porous channel within 1–2 weeks of cell culturing when metastatic M4A4-GFP breast cancer cells were induced by 20% fetal bovine serum (FBS).We also developed a mathematical model to evaluate the diffusivity and concentration gradient through the fabricated porous structure. |
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