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| bcl—2 mRNA反义寡核苷酸对胃癌SGC—7901细胞端粒酶活性的影响 | |
| 引用本文： | 范钰,林庚金,钱立平,许祖德,李华,徐康. bcl—2 mRNA反义寡核苷酸对胃癌SGC—7901细胞端粒酶活性的影响[J]. 复旦学报(医学版), 2001, 28(6): 483-485,489 |
| 作者姓名： | 范钰 林庚金 钱立平 许祖德 李华 徐康 |
| 作者单位： | 1. 复旦大学附属华山医院消化科, 2. 基础医学院病理学教研室, 3. 南通医学院附属东台医院内窥镜室, |
| 摘 要： | 目的 探讨bcl-2反义寡核苷酸（antisense phosphorothioate oligodeoxynucleotide,ASODN)对胃癌SGC－7901细胞端粒酶活性的影响。方法 应用脂质体瞬时转染法介导bcl-2反义寡核苷酸，处理人胃癌细胞系SGC－7901后，分别采用Northern blot、Western blot检测bcl-2 mRNA、蛋白表达，用端粒微孔板杂交法检测端粒酶活性。结果 bcl-2 ASODN处理的胃癌SGC－7901细胞端粒酶活性明显受到抑制，并与ASODN的浓度和处理时间有关。结论 bcl-2反义寡核苷酸可抑制胃癌细胞端粒酶活性。 |
| 关 键 词： | 反义寡核苷酸类 癌基因 细胞 端粒酶 bcl－2 胃癌 |
| 修稿时间： | 2001-05-30 |
Effects of Antisense Phosphorothioate Oligodeoxynucletides Targeted againt bcl-2 mRNA on Telomerase Activity of Gastric Cancer SGC-7901 Cell |
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| Abstract: | Purpose To study the effects of bcl 2 antisense phosphorothioate oligodeoxynucleotide,(ASODN) on telomerase activity of gastric cancer SGC?7901 cell. Methods After gastric cancer SGC?7901 cell were treated by bcl 2 ASODN,we studies the expression of bcl 2 gene using Northern blot and Western blot,and telomerase activity using telomeric repeat amplification protocol(TRAP)?microtiter plate hybridization assay. Results bcl 2 ASODN can repress telomerase activity of gastric cancer SGC?7901 cell,and the effects of repression were related to treated time and dose. Conclusions bcl 2 ASODN can repress telomerase activity of gastric cancer cell. |
| Keywords: | oligodeoxynucleotide antisense oncogenes cell teomerase bcl 2 |
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