AR-R15896AR blocks the early NMDA-induced loss of MAP2 in primary cortical cultures. |
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Authors: | N B Laferrière R Tremblay C L Murray R Monette D L Brown J P Durkin P Morley |
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Institution: | Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario. |
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Abstract: | The low-affinity use-dependent N-methyl-D-aspartate (NMDA) receptor antagonist AR-R15896AR is neuroprotective in primary rat cortical cultures exposed to toxic concentrations of NMDA and reduces the magnitude of NMDA-triggered increases in Ca2+]i. Here we show using fluorescence staining and measurements of microtubule-associated protein-2 (MAP2) levels, that AR-R15896AR inhibits the NMDA-induced loss of MAP2 that occurs within 2 min following NMDA exposure. Understanding the multiple, Ca(2+)-triggered intracellular events that occur following NMDA receptor stimulation is important to the development of safe and effective neuroprotective agents. |
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