| 筛选介导化学合成siRNA转染原代肝癌细胞的最佳试剂 |
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| 引用本文： | 王玉洁,吴韶菊,吴芹. 筛选介导化学合成siRNA转染原代肝癌细胞的最佳试剂[J]. 中国组织工程研究与临床康复, 2011, 15(2): 245-248. DOI: 10.3969/j.issn.1673-8225.2011.02.013 |
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| 作者姓名： | 王玉洁 吴韶菊 吴芹 |
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| 作者单位： | 1. 临沂师范学院生命科学学院,山东省临沂市,276005
2. 临沂市第四人民医院精神科,山东省临沂市,276005 |
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| 基金项目： | 课题受临沂师范学院立项课题 |
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| 摘 要： | 背景:理想的细胞转染试剂应具有高效安全的特点。目的:筛选能够高效介导化学合成siRNA转染原代肝癌细胞的最佳转染试剂。方法:应用Lipofectamine RNAiMAX,Lipofectamine2000和DharmaFECT1转染试剂介导FAM-siRNA和多药耐药基因MDR1siRNA转染原代肝癌细胞,分别于转染6和48h后应用流式细胞仪和实时荧光定量PCR检测转染效率,然后用MTT法检测3种转染试剂处理原代肝癌细胞24h后的细胞毒性。结果与结论:对于Lipofectamine RNAiMAX,Lipofectamine2000和DharmaFECT1转染试剂介导的FAM-siRNA和MDR1siRNA转染,流式细胞仪和实时荧光定量PCR仪检测出RNAiMAX转染效率最高(P<0.05),分别为70.3%和71.5%。MTT法检测结果表明RNAiMAX对原代肝癌细胞没有表现出细胞毒性。结果提示,由于RNAiMAX介导的FAM-siRNA和MDR1siRNA转染的效率最高,并且对细胞的毒性最小,所以RNAiMAX是最适合介导化学合成siRNA转染原代肝癌细胞的转染试剂。
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| 关 键 词： | 原代肝癌细胞 siRNA 转染 Lipofectamine RNAiMAX Lipofectamine 2000 DharmaFECT1 组织工程 |
Transfection agents screening for chemosynthesis siRNA transfection to primary liver cancer cells |
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| Wang Yu-jie,Wu Shao-ju,Wu Qin. Transfection agents screening for chemosynthesis siRNA transfection to primary liver cancer cells[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(2): 245-248. DOI: 10.3969/j.issn.1673-8225.2011.02.013 |
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| Authors: | Wang Yu-jie Wu Shao-ju Wu Qin |
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| Affiliation: | Wang Yu-jie1,Wu Shao-ju1,Wu Qin21College of Life Science,Linyi Normal University,Linyi 276005,Shandong Province,China,2the Fourth People's Hospital of Linyi City |
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| Abstract: | BACKGROUND:The ideal transfection agent should efficient and low-toxic.OBJECTIVE:To screen transfection agent which efficiently transfect chemosynthesis siRNA to primary liver cancer cells.METHODS:FAM-siRNA and MDR1 siRNA was transfected to primary liver cancer cells by Lipofectamine RNAiMAX,Lipofectamine 2000 and DharmaFECT1.The transfection efficiency was evaluated by flow cytometer and real time-PCR at 6 and 48 hours after transfection.Moreover,the cytotoxicity of transfection agents in primary liver can... |
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| Keywords: | siRNA Lipofectamine RNAiMAX Lipofectamine 2000 DharmaFECT1 |
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