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| 大鼠近交系间原位肝移植急性排斥模型的建立与评价 | |
| 引用本文： | 谢金敏,董瑞强,李涛,张会迎,姚坤厚,温浩.大鼠近交系间原位肝移植急性排斥模型的建立与评价[J].中国组织工程研究与临床康复,2011,15(5). |
| 作者姓名： | 谢金敏 董瑞强 李涛 张会迎 姚坤厚 温浩 |
| 作者单位： | 1. 新疆医科大学第一附属医院临床医学博士后流动站,新疆维吾尔自治区乌鲁木齐市,830011;解放军第四七四医院普外科,新疆维吾尔自治区乌鲁木齐市,830011 2. 石河子大学医学院,新疆维吾尔自治区石河子市,830011 3. 新疆医科大学第一附属医院临床医学博士后流动站,新疆维吾尔自治区乌鲁木齐市,830011 4. 解放军第四七四医院普外科,新疆维吾尔自治区乌鲁木齐市,830011 |
| 摘 要： | 背景:目前国内常用的封闭群品系大鼠(SD与Wistar大鼠)所建立肝移植急性排斥模型并不理想,易产生肝移植耐受.目的:通过二袖套法建立稳定的DA-Lewis大鼠原位肝移植急性排斥模型.方法:实验分为两组:同基因组:Lewis-Lewis 24例;异基因组:DA-Lewis 24例.观察移植后一般情况及移植后存活时间,两组受体分别于移植后3,5,7,10 d随机取3只处死取标本,观察肝脏组织病理变化,测定天冬氨酸转氨酶、总胆红素、细胞因子水平变化.结果与结论:同基因组大鼠无急性排斥反应表现,中位生存时间超过100 d,肝脏组织发生轻度形态学改变.异基因组大鼠移植后黄疸明显,中位生存时间为11 d,移植后第7天肝脏组织病理表现典型急性排斥反应(Banff国际标准).同期相比异基因组天冬氨酸转氨酶、总胆红素、细胞因子水平均高于同基因组(P < 0.001).提示,DA-Lewis是稳定的大鼠肝移植急性排斥模型,是研究肝移植排斥反应及免疫耐受的理想动物模型. |
| 关 键 词： | 近交系大鼠 肝移植 排斥反应 免疫耐受 动物模型 |
Establishment and evaluation of an acute rejection model of orthotopic liver transplantation in inbred rats |
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| Xie Jin-min,Dong Rui-qiang,Li Tao,Zhang Hui-ying,Yao Kun-hou,Wen Hao.Establishment and evaluation of an acute rejection model of orthotopic liver transplantation in inbred rats[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(5). | |
| Authors: | Xie Jin-min Dong Rui-qiang Li Tao Zhang Hui-ying Yao Kun-hou Wen Hao |
| Institution: | Xie Jin-min1,2,Dong Rui-qiang3,Li Tao1,Zhang Hui-ying2,Yao Kun-hou2,Wen Hao1 1The Post-doctoral Research Station,First Affiliated Hospital,Xinjiang Medical University,Urumqi 830011,Xinjiang Uighur Autonomous Region,China,2Department of General Surgery,the 474 Hospital of Chinese PLA,3Shihezi Medical University,Shihezi 834407 |
| Abstract: | BACKGROUND:The models commonly used from closed population at present were not well ideal,which easy lead to liver transplantation tolerance. OBJECTIVE:To establish a stable rat model of acute rejection following allograft liver transplantation from DA to Lewis by two-cuff method. METHODS:The rats were allocated to the isogene group (n=24,liver grafts from Lewis rat to Lewis rat) or allogene group (n=24,liver grafts from DA rat to Lewis rat). The general states and survival duration of the rats after transp... |
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