Modified photometric method for the determination of phospholipase A activities |
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Authors: | G E Hoffmann U Neumann |
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Institution: | (1) Institute of Clinical Chemistry, Bogenhausen Hospital, München;(2) Boehringer Mannheim GmbH, Tutzing |
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Abstract: | Summary A modified photometric method for the determination of phospholipase A activities which is based on a previously published reaction principle is described. The modified assay uses a lyophilized substrate emulsion rather than a freshly prepared phospholipid emulsion and a new chromogen (tribromohydroxybenzoic acid and 4-amino-antipyrine) with a high molar absorption coefficient (1,930 m2/mol at 546 nm, 2,900 m2/mol at 512 nm wavelength). The new test is more practicable with respect to pipetting volumes and incubation times. Preliminary results of a method evaluation indicate that the modified assay fulfills the usual criteria for clinical chemical enzyme measurements. |
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Keywords: | Phospholipase A Photometry Automated analysis free fatty acids |
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