以裸鼠为活体生物反应器构建可移植组织工程骨的可行性研究

目的研究以裸鼠为活体生物反应器构建组织工程骨进行自体移植的可行性。方法常规培养兔骨髓间充质干细胞后，选取第4代细胞向成骨细胞诱导分化。与珊瑚复合后，植入裸鼠体内，8局后取出形成之骨组织，放人自体家兔背部肌肉组织间隙中，分别于14、28、56d后测量血清中兔抗裸鼠抗体滴度，2、7、14、28、56d后检测白细胞介素(IL)-2水平，8周标本行免疫组化染色，判定局部免疫反应水平。结果整个实验过程中均未能测出血清IL-2活性，兔抗裸鼠抗体滴度阳性，免疫组化染色阴性。结论本实验中裸鼠体内长成的组织工程骨未表现出明显的免疫原性，提示裸鼠作为活体生物反应器构建组织工程骨有一定的可行性。

Nude mouse as in-vivo bioreactor: The feasibility study of construction transplantable tissue engineered bone

ObjectiveTo investigate the feasibility of construction transplantable tissue engineered bone in nude mouse as in vivo bioreactor. MethodsSelected the 4th passage of rabbit bone mesenchymal stem cells, which were cultured, expanded and induced in vitro. Cells were seeded into porous natural coral. The complexes were implanted subcutaneous in nude mice. Regenerated bone was taken out 8 weeks after implantation and implanted into muscle tissue interspace in the back of rabbit itself. The rabbit anti mouse antibody titer was measured at 14, 28, 56 days and the level of IL 2 was detected the at 2, 7, 14, 28 and 56 days after implantation. Immunohistochemistry staining was carried on in the 8th week. ResultsDuring the whole experimental process, IL 2 activity couldn't be measured. The rabbit anti mouse antibody titer was positive. A specific antibody reaction was detected from 2ed week to 8th week after transplantation and touches the peak value four weeks later. Immunohistochemistry staining result was negative.ConclusionThe tissue engineered bone growth in nude mouse is seemed to be the reliable transplantable bone source. [