内源性硫化氢在大鼠截肢术后肺损伤中的变化及意义

目的研究内源性血浆硫化氢（H2S）／肺胱硫醚-γ-裂解酶（CSE）体系在截肢术后肺损伤中的变化及作用。方法雄性Wistar大鼠63只，构建大鼠截肢模型，按照不同时间点随机分为9组：正常对照组7只；手术后1、2、4、6、12、24、48、72h组，各7只。观察各组大鼠肺湿／干重比值（W／D）的变化及血浆H2S、肺CSE活性的变化。结果与正常对照组肺湿／干重比（4．931±0．334）比较，术后4h，肺湿／干重比值即开始明显上升，6h到最高5．9341±0．457（P〈0．01），后逐渐下降，48h恢复正常5．051±0．251（P〉0．05）。血浆H2S水平正常值为55．654±6．334μmol／L，手术后4h，血浆H2S水平开始明显下降。6h到最低39．286±6．525μmol／L（P〈0．01），24h恢复正常50．000±8．244μmol／L（P〉0．05）。肺CSE活性正常对照组为14．006±0．720pmol·min^-1·mg^-1,术后2h明显下降，6h达最低值11．340±1．436pmol·min^-1·mg^-1（P〈0．01）；后逐渐回升，4h恢复正常14．031±0．804pmol·min^-1·mg^-1（P〉0．05）。结论截肢术后可造成肺损伤，术后6h肺湿／干重比最高。内源性血浆H2S／肺CSE体系的下调可能在创伤致肺损伤的病理生理改变中具有重要作用。

Regulatory effect of endogenous hydrogen sulfide on lung injury after amputation

Objective To investigate regulatory effect of endogenous hydrogen sulfide （H2S） on lung injury after amputation. Methods Sixty-three Wistar rats were divided into 9 groups at random：Group normal （A）, Group postopetation 1 h, 2h ,4h, 6h, 12h, 24h,48h,72h,The plasma levels of H2S,the ratio of wet over dry lung tissue weight （W/D） in each group were measured. The CSE activity in lung was detected. Results Compared with Group normal（4.931±0.334）,the level of W/D was significantly increased in Group B4 （P〈0.05）,reached peak in Group B6 5.934±0.457（P〈0.01） and recuperate at 48h 5.051±0.251 （P〉0.05）, the plasma levels of H2S were markedly decreased in Group B4 compared with Group normal（55.654±6.3341μmol/L）,reached valley bottom in Group B6 39.286±6.5251μmol/L（P〈0.01）and recuperate at 24h 50.000±8.244 μmol/L（P〉0.05）,the lung level of CSE were decreased obviously in Group B2,reached at lowest level in Group B6 11.340±1.436 pmol·min^-1·mg^-1 （P〈0.01） compared with Group normal 14.006±0.720 pmol·min^-1·mg^-1. and recuperate at 48h14.031±0.804 pmol·min^-1·mg^-1（P〉0.05）. Conclusions Amputation can induce lung injury. The ratio of wet to dry lung tissue weight （W/D） is highest in 6 hours after operation. The downregulation of endogenous H2S/CSE system may play an important role in lung injury during operation trauma.