牛膝总皂甙对大鼠离体子宫兴奋作用机理的研究

以子宫收缩面积(UCA)为指标,用消炎痛与氯丙嗪等作阻断剂,分析牛膝总皂甙(ABS)对大鼠离体子宫兴奋作用的机理。结果表明,实验前给大鼠用消炎痛(75mg/只)灌胃或浴槽内加消炎痛(20μg/ml),均可明显减弱ABS对大鼠离体子宫的兴奋作用。前列腺素E_2200ng/ml可明显增强ABS兴奋大鼠子宫作用。氯丙嗪0.5μg/ml也可明显减弱ABS对已,未孕大鼠子宫的兴奋作用,而阿托品10μg/ml对ABS所致大鼠子宫兴奋无明显影响。

STUDY OF MECHANISM OF EXCITANT ACTION OF ACHYRANTHES BIDENTATA SAPONINS ON ISOLATED RAT UTERI

0.25mg/ml of Achyranthes bidentata Saponins( ABS ) in De Jalon's solution usually caused an obvious excitant action in both virgin and pregnant uterine preparations. The uterine excitation was blocked effectively by prior treatment of the preparations with 20μg/ml of indomethacin for 1 h. In the isolated uterine preparations taken from the rats treated with indomethacin, 15 mg/rat orally twice a day for 2 and a half days, the excitant action of ABS was obviously reduced. Treatment of the uterine segments with 0.5μg/ml of chlorpromazine for half an hour also blocked the excitant response. However, treatment of the uterine preparations with 10μg/ml of atropine for 10 min had no abvious effect on the excitant response. The probable mecha-nism of action of ABS was discussed.