| MDM2-P53反馈调节对大肠癌作用的临床病理学研究 |
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| 引用本文： | 成志强,王晓玫,单军,高利昆,许静,彭全洲,胡锦涛.MDM2-P53反馈调节对大肠癌作用的临床病理学研究[J].罕少疾病杂志,2010,17(4):1-6. |
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| 作者姓名： | 成志强 王晓玫 单军 高利昆 许静 彭全洲 胡锦涛 |
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| 作者单位： | 1. 深圳市人民医院病理科,广东,深圳,518020
2. 深圳市人民医院放射科 |
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| 摘 要： | 目的分析MDM2基因扩增、P53基因缺失与蛋白异常表达在大肠癌发生演进中的作用、与临床病理因素的关系。方法应用RT-PCR、FISH和免疫组化技术,检测72例大肠癌组织MDM2基因扩增、p53基因缺失以及其蛋白表达特点。结果大肠癌MDM2基因扩增、p53基因缺失率为28.1%和53.1%,两蛋白表达阳性率为43.0%和65.0%。MDM2、p53蛋白在肝转移、淋巴结转移组中表达明显增多,与对照组比较差异有显著性（P=0.011）;p53基因缺失、MDM2基因扩增在有静脉侵袭大肠癌组明显高于无静脉侵袭组（60.7%和31.8%）,MDM2蛋白表达与癌细胞对静脉侵袭性有关（P=0.016）。结论 MDM2、p53基因参与大肠癌发生发展过程,MDM2在肝转移组、静脉侵袭组表达明显增多,可作为肝内微小转移的参考指标,MDM2、p53蛋白表达与淋巴结转移、肝转移有明显相关性,提示预后不良,两基因联合检测对大肠癌预后评估有重要意义。
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| 关 键 词： | MDM2癌基因 p53抑癌基因 大肠癌 荧光原位杂交 RT-PCR |
A clinicopathological study of the Relationship of MDM2 and P53 gene with biological behavior in colorectal carcinoma |
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| CHENG Zhi-qiang,WANG Xiao-mei,SHAN Jun,et al..A clinicopathological study of the Relationship of MDM2 and P53 gene with biological behavior in colorectal carcinoma[J].Journal of Rare and Uncommon Diseases,2010,17(4):1-6. |
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| Authors: | CHENG Zhi-qiang WANG Xiao-mei SHAN Jun |
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| Institution: | CHENG Zhi-qiang1,WANG Xiao-mei1,SHAN Jun2,et al.Department of Pathology1 and Department of Radiology2,Shenzhen People's Hospital.Shenzhen,518020,China. |
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| Abstract: | Objective To investigate the relationship of MDM2 gene,P53 gene with clinicopathologic parameters in colorectal carcinoma.Methods The detection of MDM2 amplification,p53 deletion and their protein in 72 patients by RT-PCR,FISH and immunohistochemical technique.Results The ratio of MDM2 and P53 gene expression in the group of the colorectal carcinoma was 28.1% and 53.1%,but protein expression was 43.0% and 65.0%,respectively.Expression of MDM2 and p53 protein was higher in the groups of lymph node metastasis... |
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| Keywords: | RT-PCR |
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