| 雌激素对前成骨细胞胰岛素受体底物-1的影响 |
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| 引用本文： | 何玉玲,周后德,隋国良,谢辉,廖二元. 雌激素对前成骨细胞胰岛素受体底物-1的影响[J]. 广西医科大学学报, 2012, 29(3): 333-337 |
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| 作者姓名： | 何玉玲 周后德 隋国良 谢辉 廖二元 |
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| 作者单位： | 何玉玲 (广西医科大学第一附属医院代谢糖尿病中心,南宁,530021) ； 周后德 (中南大学湘雅二医院代谢内分泌研究所,长沙,410011) ； 隋国良 (中南大学湘雅二医院代谢内分泌研究所,长沙,410011) ； 谢辉 (中南大学湘雅二医院代谢内分泌研究所,长沙,410011) ； 廖二元 (中南大学湘雅二医院代谢内分泌研究所,长沙,410011) ； |
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| 基金项目： | ,国家自然科学基金资助项目, |
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| 摘 要： | 目的:观察前成骨样细胞(MC3T3-E1细胞)分化过程中17β-雌二醇对胰岛素受体底物-1(insulin receptor substrate-1,IRS-1)的影响。方法:应用10mmol/Lβ-甘油磷酸钠和50mg/L抗坏血酸诱导小鼠MC3T3-E1细胞分化成熟,分别在0,6,12,18,24,30d收集细胞。17β-雌二醇干预,用半定量RT-PCR和免疫印迹法检测干预组和对照组细胞IRS-1mRNA和蛋白的表达,比较两组细胞IRS-1表达量的变化。结果:在MC3T3-E1细胞分化成熟过程中,IRS-1 mRNA和蛋白的表达在MC3T3-E1细胞分化成熟过程中逐渐增高。结论:IRS-1可能在17β-雌二醇诱导的骨转换过程中起着重要作用。
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| 关 键 词： | MC3T3-E1细胞 胰岛素受体底物-1 17β-雌二醇 |
EFFECT OF ESTRADIOL ON THE EXPRESSION PROFILE OF IRS-1 DURING MC3T3-E1 CELLS DIFFERENTIATION |
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| He Yuling,Zhou Houde,Sui Guoliang,Xie Hui,Liao Eryuan. EFFECT OF ESTRADIOL ON THE EXPRESSION PROFILE OF IRS-1 DURING MC3T3-E1 CELLS DIFFERENTIATION[J]. Journal of Guangxi Medical University, 2012, 29(3): 333-337 |
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| Authors: | He Yuling Zhou Houde Sui Guoliang Xie Hui Liao Eryuan |
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| Affiliation: | .(The Diabetes Mellitus and Metabolism Research Center,the First Affiliated Hospital of Guangxi Medical University,Nanning,530021,China) |
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| Abstract: | Objective:To observe the effect of estradiol on the expression profile of insulin receptor substrate-1(IRS-1) during murine preosteoblastic MC3T3-E1 cells differentiation.Methods: MC3T3-E1 cell was cultured and underwent intervention of 17β-estradial.Cells were collected respectively in days 0,6,12,18,24 and 30.Semi-quantitative RT-PCR was used to measure the expression of mRNA of IRS-1.The expression of the proteins of IRS-1 was detected by Western blotting.Results: IRS-1 mRNA and protein levels gradually increased during differentiation of MC3T3-E1 cells.Estradiol increased IRS-1 mRNA and protein expressions in the whole time course.Conclusion: IRS-1 may play an important role in the process of estradiol inducing bone turnover. |
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| Keywords: | murine preosteoblastic MC3T3-E1 cells insulin receptor substrate-1 17β-estradial |
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