盐酸戊乙奎醚后处理对心肌缺血-再灌注大鼠心功能的影响

目的探讨盐酸戊乙奎醚后处理对心肌缺血-再灌注(IR)大鼠心功能的影响。方法选择SPF级健康雄性Wistar大鼠32只,8周龄,体重220~250 g。将大鼠随机分为四组:假手术组(S组)、假手术+盐酸戊乙奎醚组(SP组)、IR组和IR+盐酸戊乙奎醚后处理组(IP组),每组8只。S组冠状动脉左前降支仅穿线不结扎,30 min后经尾静脉注射生理盐水1 mg/kg。SP组冠状动脉左前降支仅穿线不结扎,30 min后经尾静脉注射盐酸戊乙奎醚1 mg/kg。IR组冠状动脉左前降支穿线结扎30 min后,松开结扎线,再灌注3 h。于再灌注(松开结扎线)前即刻经尾静脉注射生理盐水1 mg/kg。IP组于再灌注前即刻经尾静脉注射盐酸戊乙奎醚1 mg/kg。于开胸前5 min、再灌注后3、6 h,采用经胸超声心动图监测大鼠左心室舒张末压(LVEDP)、左心室收缩末压(LVESP)、左心室射血分数(LVEF)、左心室缩短分数(LVFS),采用智能血压计监测大鼠尾动脉MAP,采用ELISA法检测血清肌钙蛋白T(cTnT)浓度。结果与开胸前5 min比较,再灌注后3、6 h IR组和IP组LVEDP明显升高,LVESP、LVEF、LVFS、MAP明显降低(P<0.05);再灌注后3、6 h S组、IR组和IP组血清cTnT浓度明显升高(P<0.05)。与再灌注后3 h比较,再灌注后6 h IR组LVEDP、血清cTnT浓度明显升高,LVESP、LVEF、LVFS、MAP明显降低(P<0.05)。与S组比较,再灌注后3、6 h IR组和IP组LVEDP、血清cTnT浓度明显升高,LVESP、LVEF、LVFS、MAP明显降低(P<0.05)。与IR组比较,再灌注后3、6 h IP组LVEDP、血清cTnT浓度明显降低,LVESP、LVEF、LVFS、MAP明显升高(P<0.05)。S组和SP组上述指标差异均无统计学意义。结论盐酸戊乙奎醚后处理能够维持血流动力学稳定,降低cTnT浓度,改善心肌缺血-再灌注大鼠的心功能。

Effects of penehyclidine hydrochloride postconditioning on cardiac function in rats with myocardial ischemia-reperfusion

Objective To investigate the effects of penehyclidine hydrochloride postconditioning on cardiac function in myocardial ischemia-reperfusion (IR) rats.
Methods Thirty-two SPF healthy male Wistar rats, aged 8 weeks, weighing 220-250 g, were randomly divided into four groups: sham operation group (group S), sham operation combined with penehyclidine hydrochloride group (group SP), IR group (group IR), and IR combined with penehyclidine hydrochloride postconditioning group (group IP), 8 rats in each group. In group S, the left anterior descending coronary artery was only threaded without ligation, and normal saline 1 mg/kg was injected into the caudal vein 30 minutes later. In group SP, the left anterior descending coronary artery was only threaded without ligation, and penehyclidine hydrochloride 1 mg/kg was injected into the caudal vein 30 minutes later. In group IR, the left anterior descending coronary artery was ligated for 30 minutes, the ligation thread was loosened and reperfusion for 3 hours, and normal saline 1 mg/kg was injected into the caudal vein immediately before reperfusion (the ligation thread was loosened). In group IP, penehyclidine hydrochloride 1 mg/kg was injected into caudal vein immediately before release and reperfusion. Left ventricular end-diastolic pressure (LVEDP), left ventricular end-systolic pressure (LVESP), left ventricular ejection fraction (LVEF), and left ventricular shortening fraction (LVFS) were measured by transthoracic echocardiography, MAP of caudal artery was monitored with smart sphygmomanometer, the concentration of serum troponin T (cTnT) was detected by ELISA 5 minutes before chest opening, 3 hours after reperfusion and 6 hours after reperfusion.
Results Compared with 5 minutes before chest opening, LVEDP in groups IR and IP significantly increased 3 and 6 hours after reperfusion, LVESP, LVEF, LVFS, and MAP decreased significantly (P < 0.05); cTnT increased significantly in groups S, IR and IP 3 and 6 hours after reperfusion (P < 0.05). Compared with 3 hours after reperfusion, LVEDP and cTnT in group IR increased significantly 6 hours after reperfusion, LVESP, LVEF, LVFS, and MAP decreased significantly (P < 0.05). Compared with group S, LVEDP and cTnT in groups IR and IP were significantly higher 3 and 6 hours after reperfusion (P < 0.05), LVESP, LVEF, LVFS, and MAP were significantly lower (P < 0.05). Compared with group IR, LVEDP and cTnT in group IP were significantly lower 3 and 6 hours after reperfusion (P < 0.05), LVESP, LVEF, LVFS, and MAP were significantly higher (P < 0.05). There were no significantly differences between groups S and SP.
Conclusion Penehyclidine hydrochloride postconditioning can maintain hemodynamic stability, reduce cTnT concentration and improve cardiac function in rats with myocardial ischemia-reperfusion.