他克莫司联合白藜芦醇对同种异体心脏移植术后T细胞亚群及免疫功能的影响

目的研究他克莫司（FK506）联合白藜芦醇（resveratrol，Res）对同种异体心脏移植术后急性排异反应的抑制作用。方法采用大鼠异体异位移植模型，将心脏移植于腹部。术后分别进行不同免疫治疗，于移植术后第7d检测外周血T细胞亚群分布和血清IL-2含量，同时观察心脏移植术后移植心脏的病理改变。实验分单纯应用FK506组、单纯应用Res组以及联合应用FK506和Res组，以异体异位移植术后不用药为对照组。结果单独用药组与不用药对照组比较，移植心脏病理改变明显减轻，联合用药组又明显轻于单独用药组。各用药组外周血CD4^＋T淋巴细胞明显低于未用药组。结论FK506和Res都可以通过抑制CD4^＋T淋巴细胞增殖而阻止或延缓同种异体心脏移植的急性排异反应。FK506与Res联用具有协同作用。可更有效地产生免疫抑制作用。

Effect of FKS06 combinated with resveratrol on T-cell subsets and its immune function following homologous heart transplantation

[Objective] To investigate the preventive effects of FK506 combinated with resveratrol on acute rejection following homologous heart transplantation. [Methods] Donor's heart derived from rat was transplanted into the abdomen cavity of another rat. On the 7th day after transplantation, CD4+, CD8+T-cell subsets and IL-2 were examined, and graft's pathological changes were observed. The recipients were randomly divided into mono-FK506 therapy group, mono- resveratrol therapy group and combination-therapy group, the un-treatment group as control. [Results] Compared with un-treatment group, graft's pathological changes were significantly prolonged in mono-therapy group; Combination-therapy of FK506 with resveratrol could significantly prolong the survival of xen-graft heart when compared with FK506 or resveratrol used alone. The number of CD4+T-cell and level of IL-2 in mono-therapy group and combination-therapy group were much less than in un-treatment group. [Conclusion] Both FK506 and resveratrol can suppress proliferation of CD4+T-cell and expression level of IL-2, so they can prevent or delay acute rejection.