冬虫夏草及其混淆品HPLC指纹图谱研究及共有成分鉴定

目的建立冬虫夏草水溶性成分的HPLC指纹图谱方法,为冬虫夏草的真伪鉴别及质量评价提供依据。方法采用HPLC法建立冬虫夏草及其主要混淆品(凉山虫草、古尼虫草、蛹虫草)水溶性成分的指纹图谱,标记了16个共有峰,并通过超高效液相色谱-四级杆飞行时间质谱(UPLC/Q-TOF-MS)技术对指纹图谱中16个共有峰进行鉴定。结果建立了冬虫夏草水溶性成分的HPLC指纹图谱方法,该方法能区分冬虫夏草及其混淆品,鉴定出其中12个共有峰,分别为胞嘧啶、尿嘧啶、胞苷、次黄嘌呤+鸟嘌呤、尿苷、胸腺嘧啶、腺嘌呤、肌苷、鸟苷、胸苷、腺苷和虫草素。结论该方法简便易行,为冬虫夏草的真伪鉴别提供了科学依据。

HPLC fingerprint of Cordyceps sinensis and its confused species and identification of common composition

Objective To establish an HPLC fingerprint method for water-soluble components in Cordyceps sinensis which could provide the basis for authenticity identification and quality evaluation of C. sinensis. Methods HPLC method was adopted to construct the fingerprint of water soluble components in C. sinensis and its main confused species (C. liangshanensis, C. gunnii and artificial C. militaris). Total 16 common peaks were marked, and 12 common peaks were confirmed by the method with tandem high performance liquid chromatography (HPLC) and quadrupole time-of-flight mass spectrometry (Q-TOF MS). Results The common mode of HPLC fingerprint for C. sinensis was developed. The method could distinguish between C. sinensis and its confused species, and 12 common peaks were identified, including cytosine, uracil, cytidine, hypoxanthine+guanine, uridine, thymine, adenine, inosine, guanosine, thymidine, adenosine, and cordycepin respectively. Conclusion This method is simple and easy, providing scientific basis for identification of C. sinensis.