肠缺血/再灌注损伤时中性粒细胞呼吸爆发活性的变化

目的:研究肠缺血/再灌注(I/R)时中性粒细胞(PMN)呼吸爆发活性的变化.方法:30只成年雄性Wistar大鼠,按照随机数字表法分为假手术,缺血45 min,缺血45 min再灌注60min、120min、360min等5组.阻断肠系膜上动脉血流45min后复流,复制I/R模型:假手术组只进行同样的手术操作但不阻断肠系膜上动脉血流.在各时间点分别取门静脉血测定白细胞计数,并分离PMN进行化学发光(CL)测定.结果:肠I/R损伤过程中,缺血组与假手术组比较,PMN的CL峰值无明显差异(P＞0.05),再灌注组PMN的CL峰值均明显升高(P＜0.01).肠缺血组白细胞数量较假手术组降低,再灌注后开始回升,再灌注360 min时白细胞计数最高.PMN化学发光峰值变化和血白细胞计数的变化趋势呈显著正相关(r=0.748,P＜0.05).结论:肠I/R损伤可激活循环中的PMN,使血中的PMN数量增加,PMN的呼吸爆发化学发光活性明显升高,可能是引起全身炎症反应和器官损害的因素之一.

Change in respiratory burst of polymorphonuclear neutrophils in rats with intestine ischemia/reperfusion injury

Objective:To investigate the important role in chemiluminescence(CL)intensity of polymorphonuclear neutrophils(PMN)during respiratory burst and leukocyte count in rats subjected to ischemia/reperfusion(I/R)injury of intestine.Methods:Thirty Wistar male rats were randomly divided into following groups:sham operation,45 minutes-intestine ischemia,45 minutes-intestine ischemia followed by reperfusion for 60 minutes,120 minutes and 360 minutes .The superior mesenteric artery(SMA)was blocked for 45 minutes and then SMA blood flow was restored to produce I/R injury.At different time points after I/R injury,the leukocyte count and CL of PMN were determined.Results:Compared to the sham-operated group,no significant difference was found in the peak level of PMN CL in ischemia group,but it was enhanced after reperfusion(all P<0.01).At 45 minutes after intestine ischemia,leukocyte count lowered,but it rose after reperfusion of intestine,and reached the peak level at 360 minutes after reperfusion.The changes in the peak level of PML CL were positively correlated with the changes in blood leukocyte count(r=0.748,P<0.05).Conclusion:Intestinal I/R injury could increase the quantity of circulatory PMN and enhance the activity of PMN respiratory burst,which might lead to systemic inflammatory response and organ injury.