男性泌尿生殖道感染患者分离奈瑟菌的16S rDNA和PIA基因检测

目的检测男性泌尿生殖道分离奈瑟菌的16SrDNA和PIA基因,探讨奈瑟菌属菌种的基因鉴定及其致病机理。方法用PCR扩增和核苷酸序列分析方法分别检测11例男性泌尿生殖道感染患者泌尿生殖道分离的14株奈瑟菌属菌种的16SrDNA和PIA基因及其序列。结果 14株奈瑟菌属菌种经16SrDNA检测鉴定分别为淋病奈瑟菌2株,黏液奈瑟菌3株,灰色奈瑟菌5株,微黄奈瑟菌2株,干燥奈瑟菌1株,嗜乳糖奈瑟菌及多糖奈瑟菌各1株;与常规细菌学方法鉴定的符合率为85.7%。非淋球菌奈瑟菌未检出淋病奈瑟菌毒力相关的PIA核苷酸序列。结论常规细菌学方法与染色体16SrDNA检测及其序列分析方法的联合使用,可提高奈瑟菌属菌种感染的实验室诊断准确率;PIA基因对于奈瑟菌属的男性生殖道致病性无关。

Detection of 16S rDNA and PIA genes of Neisseria isolated from the patients with male genitourinary tract infections

Objective To detect the 16S rDNA and PIA genes of Neisseria isolated from the patients with male genitourinary tract infections and investigate the gene identification and pathogenesis of Neisseria species.Methods The 16S rRNA and PIA genes and thier sequences of 14 Neisseria species isolated from reproductive tract of 11 patients with male genitourinary tract infections were identified by PCR amplification and nucleotide sequencing.Results Fourteen Neisseria strains contained 2 strains of Neisseria gonorrhoeae,3 strains of Neisseria mucosa,5 strains of Neisseria cinerea,2 strain of Neisseria subflava,1 strain of Neisseria sicca,1 strain of Neisseria lactamica and 1 strain of Neisseria polysaccharea.The accordance rate of the results reported by 16S rDNA and routine bacteriological method was 85.7%.The nucleotide sequence of virulence-associated gene PIA of Neisseria gonorrhoeae was not found in non-gonococcal strains of Neisseria.ConclusionThe accuracy rate of laboratory diagnosis for the infection caused by Neisseria can be improved by combined use of routine bacteriological method and 16S rDNA identification.The PIA gene is not responsible for the pathogenicity of Neisseria species that cause male reproductive tract infection.