| Drosha基因在人卵巢癌顺铂耐药细胞中的表达研究 |
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| 引用本文： | 况燕,蔡晶,李桃,李东林,周隽,王泽华. Drosha基因在人卵巢癌顺铂耐药细胞中的表达研究[J]. 广西医学, 2012, 34(7): 819-821 |
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| 作者姓名： | 况燕 蔡晶 李桃 李东林 周隽 王泽华 |
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| 作者单位： | 1. 广西医科大学第一附属医院妇产科,南宁市,530021
2. 华中科技大学同济医学院附属协和医院妇产科,武汉市,430022 |
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| 基金项目： | 湖北省卫生厅科学研究基金 |
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| 摘 要： | 目的 探讨Drosha基因和卵巢癌顺铂耐药的关系.方法 分别采用实时定量PCR和免疫印迹法检测Drosha基因mRNA及其蛋白在A2780/DDP及A2780细胞中表达的差异.结果 A2780/DDP细胞中Drosha基因mRNA及其蛋白的表达量分别是A2780细胞表达量的(56.79±1.65)%和(50.16±1.34)%,两者比较差异均有统计学意义(P＜0.001).结论 Drosha基因在A2780/DDP细胞中明显下调,提示Drosha基因和卵巢癌顺铂耐药相关.
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| 关 键 词： | 卵巢肿瘤 耐药性 Drosha基因 |
Expression of Drosha Gene in Cisplatin-resistant Ovarian Cell Line A2780/DDP |
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| KUANG Yan , CAI Jin , LI Tao , LIN Dong-lin , ZHOU Jun , WANG Ze-hua. Expression of Drosha Gene in Cisplatin-resistant Ovarian Cell Line A2780/DDP[J]. Guangxi Medical Journal, 2012, 34(7): 819-821 |
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| Authors: | KUANG Yan CAI Jin LI Tao LIN Dong-lin ZHOU Jun WANG Ze-hua |
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| Affiliation: | 1 Department of Obstetrics and Gynecology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;2 Department of Obstetrics and Gynecology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China) |
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| Abstract: | Objective To investigate the relationship between Drosha gene and cisplatin(DDP)-resistance in ovarian cancer.Methods The mRNA and protein expression of Drosha gene in A2780/DDP and A2780 were detected by real-time quantitative RT-PCR and Western blot assay,respectively.Results The expression of mRNA and protein of Drosha gene in cisplatin-resistant ovarian cancer cell line A2780/DDP were(56.79±1.65)% and(50.16±1.34)%,respectively,and the differences were significant compared with those in ovarian cancer cell line A2780(P<0.001).Conclusion The expression of Drosha gene is down-regulated in A2780/DDP cells,which suggests that Drosha gene is correlated with cisplatin-resistance in ovarian cancer. |
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| Keywords: | Ovarian neoplasm Drug resistance Drosha gene |
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