Ontogeny of GABA-immunoreactive cells in the primate cerebellar cortex: comparison with somatostatin-immunoreactivity

The distribution and ontogeny of GABA-immunoreactive cells were studied and compared with that of somatostatin-immunoreactivity in the primate cerebellar cortex. At embryonic day 80, we observed neither GABA-nor somatostatin-immunoreactive cells in the cerebellum. At embryonic day 110, a small number of GABA-immunoreactive cells was detectable in the granular layer only, and these cells seemed to be Golgi cells. At embryonic day 140, although almost all Purkinje cells were somatostatin-immunoreactive, a proportion of these cells was GABA-immunoreactive. At the newborn stage, most of the Purkinje cells were GABA-immunoreactive and almost all of them were also somatostatin-immunoreactive. During the postnatal stages, the number of somatostatin-immunoreactive cells decreased until postnatal day 60. At the adult stage, a large number of Purkinje cell bodies was faintly GABA-immunoreactive and a proportion of Purkinje cell dendrites was GABA-immunoreactive. In the aged animals (28 and 31 years old), a small number of Purkinje cell dendrites was GABA-immunoreactive. These findings suggest that a transition of phenotype from somatostatin to GABA occurred in Purkinje cells during development.Abbreviations Ad Adult - EX embryonic day X - GABA -aminobutyric acid - GAD glutamic acid decarboxylase - ir immunoreactive - Nb newborn (postnatal day 1–3) - NGF nerve growth factor - PX postnatal day X - PXy X years old - P8y < more than 8 years old - PBS phosphate-buffered saline - PL Purkinje cell layer - SOM somatostatin - WM white matter