胆管癌组织紧密连结蛋白18、丝氨酸蛋白酶抑制剂和p53蛋白表达及其临床意义

目的 探讨胆管癌组织紧密连结蛋白18（claudin-18）、丝氨酸蛋白酶抑制剂（maspin）和p53蛋白表达的临床意义。方法 采用免疫组化和Western blot法检测46例胆管癌和32例正常胆管组织claudin-18、maspin和p53蛋白的表达,采用Spearman等级相关分析。结果 胆管癌组织claudin-18和p53蛋白阳性率分别为52.17%和56.52%,显著高于正常胆管组织（0.0%和0.0%,P<0.01）;胆管癌组织maspin阳性率为86.96%,显著低于正常胆管组织（100.0%,P<0.01）;胆管癌组织claudin-18、maspin和p53蛋白表达量分别为2.83±0.52、2.45±1.24和1.73±1.26,均显著高于正常胆管组织（0.48±0.75、0.46±0.32和0.0±0.0,P<0.05）;胆管癌组织maspin与p53表达呈负相关（r=-8.546,P=0.001）。结论 胆管癌组织maspin、claudin-18和p53蛋白表达的意义还有待于进一步研究。

Expression of claudin-18, maspin and p53 in tissues from cholangiocarcinoma

Objective To study the expression of claudin-18,maspin and p53 in tissues of cholangiocarcinoma. Methods The expression of claudin-18,maspin and p53 were detected by immunohistochemistry and Western blot in 46 cholangiocarcinoma and 32 normal liver tissues. Result The positive rates of claudin-18 and p53 in cancerous tissues were 52.17% and 56.52%,respectively,significantly higher than 0.0% and 0.0% in normal control（P<0.01）;The positive rate of maspin in cancerous tissues was 86.96%,much lower than 100.0% in normal control（P<0.01）;The relative expression levels of claudin-18,maspin and p53 were 2.83±0.52, 2.45±1.24 and 1.73±1.26, respectively,significantly higher than 0.48±0.75,0.46±0.32 and 0.0±0.0 in the control （P<0.05）;The expression of maspin was negatively correlated with p53 expression in cancerous tissues（r=-8.546,P=0.001）. Conclusion The implication of maspin,claudin-18 and p53 expression in cholangiocarcinoma tissues needs further investigation.