Isolation,sequencing, and characterization of crp-5, a gene encoding a Neurospora ribosomal protein |
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| Authors: | Zihua Wang Khaled A Tarawneh Stephen J Free |
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| Institution: | (1) Department of Biological Sciences, State University of New York at Buffalo, 14260 Buffalo, NY, USA;(2) Present address: Department of Biology, Mutah University, Mutah-Karak, Jordan |
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| Abstract: | A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences showed that they contain an open reading frame which encodes a protein homologous to the rat ribosomal protein S26. The crp-5 mRNA levels are regulated in a carbon-source-dependent manner. The organization of the gene and the region upstream of the coding sequences are discussed. |
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| Keywords: | Ribosomal protein gene crp-5 Neurospora crassa |
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