无热量超短波对脑缺血再灌注损伤大鼠早期CoQ10和C1GALT1C1表达的影响

目的： 探讨脑缺血再灌注损伤大鼠在不同时间点予以无热量超短波(ultrashort wave，USW)治疗后脑内辅酶Q10(coenzyme Q10，CoQ10)、β1,3-半乳糖基转移酶-特异性伴侣1(β1,3-galactosyl transferase specific chaperone 1，C1GALT1C1)表达水平的变化趋势及其对缺血性脑损伤的保护机制。方法： 50 只Sprague-Dawley 大鼠随机分为5 组，每组10 只。1 组是作为对照的假手术组，线栓插入深度为1 cm；其余4 组为实验组(分别为模型1 d 组、USW1 d 组、模型3 d 组、USW3 d 组)，线栓插入深度为18 mm，2 h 后予以再灌注。4 个实验组中，每组随机选取5 只大鼠行盐酸2,3,5-三苯基四氮(2,3,5-triphenyltetrazoliumchloride，TTC)染色，其余5 只大鼠行蛋白质印迹法和real-time PCR检测，观察比较各组大鼠脑梗死体积百分比值和缺血侧大脑中CoQ10 和C1GALT1C1 的相对表达量。结果： TTC染色后所得脑梗死体积百分比值中，假手术组未见脑梗死，比值为0；实验组随着病程延长和USW治疗呈下降趋势，差异均有统计学意义(均P<0.05)。蛋白质印迹法和real-time PCR 检测显示：假手术组CoQ10 相对表达量最高，但实验组CoQ10 相对表达量随着病程延长和USW治疗呈上升趋势，差异均有统计学意义(均P<0.05)；假手术组C1GALT1C1的相对表达量最低，但实验组C1GALT1C1 的相对表达量随着病程延长和USW治疗呈下降趋势，差异均有统计学意义(均P<0.05)。结论： 无热量USW治疗脑缺血再灌注损伤大鼠，可能通过上调CoQ10 表达及下调C1GALT1C1 表达而发挥保护作用。

Effects of non-caloric ultrashort wave on the expression of CoQ10 and C1GALT1C1 in rats with cerebral ischemia reperfusion injury

Objective: To examine the changes of coenzyme Q10 (CoQ10) and β1,3-galactosyltransferase specific chaperone 1 (C1GALT1C1) in brain of rats with ischemic injury atdifferent time points and to explore the protective mechanism of ultrashort wave (USW) onischemic brain injury.Methods: Fifty SD rats were randomly divided into 5 groups (n=10 per group): a shamgroup (control group) and 4 experimental group (ischemia for 2 h). The 4 experimentalgroups were set as a model 1 d group, a USW 1 d group, a model 3 d group and a USW 3 dgroup, respectively. Five rats were randomly selected for 2,3,5-triphenyltetrazoliumchloride(TTC) staining in each experimental group, and the remaining 5 rats were subjected toWestern blotting and real-time PCR. The percentage of cerebral infarction volume and therelative expression level of CoQ10 and C1GALT1C1 in the brain were examined andcompared.Results: The infarct volume percentage after TTC staining was zero in the sham group.With the progress of disease and USW therapy, the infarct volume percentage wasdecreased in the experimental groups (all P<0.05); Western blotting and real-time PCRshowed that the relative expression level of CoQ10 in the sham group was the highest,while in the experimental groups, the content of CoQ10 showed a upward trend with theextension of disease and USW therapy, with significant difference (all P<0.05). Therelative expression level of C1GALT1C1 in the sham group was the lowest, but in theexperimental groups, they showed a downward trend with the extension of disease andUSW therapy, with significant difference (all P<0.05).Conclusion: Non-caloric USW therapy may upregulate the expression of CoQ10 tosuppress the expression of C1GALT1C1 in rats, leading to alleviating cerebral ischemicreperfusion injury.