| 程控降温后液氮保存对自体外周血造血干细胞含量和活性的影响 |
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| 引用本文： | 路继红,欧英贤,白海,魏亚明,王存邦,吕桂玲. 程控降温后液氮保存对自体外周血造血干细胞含量和活性的影响[J]. 西北国防医学杂志, 2001, 22(3): 245-247 |
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| 作者姓名： | 路继红 欧英贤 白海 魏亚明 王存邦 吕桂玲 |
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| 作者单位： | 兰州军区兰州总医院血液科, |
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| 摘 要： | 目的：观察程控降温后液氮保存对自体外周血干细胞含量和活性的影响。方法：13例接受APBSCT患者分离的造血干细胞利用程控降温仪进行冷冻，然后置液氮液相内（－196℃）保存，检测患者冻存前后的有核细胞数，CFU－GM，CD34＋细胞。结果：冻存前后CFU－GM，有核细胞数，CD34＋细胞统计学之间无显著性差异。结论：自体外周血干细胞体外冷冻处理和保存是有效和适当的。
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| 关 键 词： | 造血干细胞 自体外周血干细胞 液氮保存 程控降温 APBSCT |
| 文章编号： | 1007-8622(2001)03-0245-03 |
| 修稿时间： | 2000-12-26 |
The effects of programmed freezing and liquid nitrogen storage on stem cells quantity and vitality |
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| LU Ji hong,OU Ying xian,Bai Hai,et al.. The effects of programmed freezing and liquid nitrogen storage on stem cells quantity and vitality[J]. Medical Journal of National Defending Forces in Northwest China, 2001, 22(3): 245-247 |
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| Authors: | LU Ji hong OU Ying xian Bai Hai et al. |
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| Affiliation: | LU Ji hong,OU Ying xian,Bai Hai,et al . |
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| Abstract: | Objective:To investigate effects of programmed freezing and liguid nitrogen storage on number of auto-peripheral stem cells and their vitality.Methods:Auto-peripheral stem cells from 13 APBSCT recipients were programmatical freezed and stored in liquid nitrogen, the mononudeus cells (MNC) CFU GM CD34 cells were examined as well.Results:MNC,CFU GM,CD34 cells showed no difference between pre and post freezing.Conclusion:Pregrammed freezing and liguid nitrogen storage of stem cells are effective and suitable for APBSCT. |
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| Keywords: | Stem cell Auto-peripheral stem cell Liquid nitrogen storage Programmed freezing |
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