| 喉鳞状细胞癌中微卫星的异常改变 |
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| 引用本文： | 陈菲菲,祝威,刘冰,于红,阮洋,张园丁. 喉鳞状细胞癌中微卫星的异常改变[J]. 临床耳鼻咽喉头颈外科杂志, 2009, 23(6): 241-244 |
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| 作者姓名： | 陈菲菲 祝威 刘冰 于红 阮洋 张园丁 |
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| 作者单位： | 1. 吉林大学第一临床医院耳鼻咽喉-头颈外科,长春,130021
2. 吉林大学基础医学院医学遗传教研室 |
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| 基金项目： | 吉林省科学技术委员会资助项目 |
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| 摘 要： | 目的：从分子生物学水平探究微卫星的不稳定性（MSI）与杂合性缺失（LOH）在喉鳞状细胞癌发病机制中的意义。方法：选择3号，5号及11号染色体的3个微卫星位点采用PCR和聚丙烯酰胺凝胶电泳-硝酸银染色方法对40例喉鳞状细胞癌患者手术切除的癌组织及癌旁正常组织进行微卫星分析。结果：40例喉鳞状细胞癌中，35例（87．5％）分别有1～3个微卫星位点发生MSI或LOH。微卫星异常改变发生率最高的位点为D5s592，占70％（28／40）；其次是D3s1228位点，占52．5％（21／40）。结论：在3p14区域及5q23区域附近的抑癌基因参与致癌机制，D3s1228和D5s592的微卫星改变与喉鳞状细胞癌的临床分期相关。
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| 关 键 词： | 喉肿瘤 微卫星的不稳定性 杂合性缺失 抑癌基因 |
Microsatellite alternation in laryngeal squamous cell carcinomas |
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| CHEN Feifei,ZHU Wei,LIU Bing,YU Hong,RUAN Yang,ZHANG Yuanding. Microsatellite alternation in laryngeal squamous cell carcinomas[J]. Journal of clinical otorhinolaryngology, head, and neck surgery, 2009, 23(6): 241-244 |
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| Authors: | CHEN Feifei ZHU Wei LIU Bing YU Hong RUAN Yang ZHANG Yuanding |
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| Affiliation: | 1Department of Otorhinolaryngology-Head and Neck Surgery;the First Hospital of Medical College;Jilin University;Changchun;130021;China;2Department of Medical Genetics;the Basic Medical College;Jilin University |
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| Abstract: | Objective:To examine the microsatellite instability and loss of heterozygosity in the pathogenic mechanism of laryngeal squamous cell carcinomas.Method:Forty cases squamous cell carcinomas of larynx were analyzed by comparing tumorous tissues and normal tissues around with 3 microsatellite markers from chromosome 3,5 and 11,using PCR and PGE-AgNO3 staining.Result:Among the 40 cases of laryngeal squamous cell carcinomas,87.5%(35/40)of samples showed microsatellite instability or loss of heterozygosity in one... |
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| Keywords: | laryngeal neoplasms microsatellitc instability loss of heterozygosity suppressor genes |
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