| Cytotoxicity of Benzo(a)pyrene-7,8-dihydrodiol on Genetically Enginered V79 Chinese Hamster Cells Expresing Rat Liver Cytochrome P450 1A1 |
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| 作者姓名： | 崔景荣 |
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| 作者单位： | 北京医科大学天然药物及仿生药物国家重点实验室，荷兰自由大学化学系药物化学室分子毒理组 |
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| 摘 要： | 本文采用细胞计数和中性红摄取法观察了ＢＰ７，８ｄｉｏｌ对ＸＥＭ２（稳定表达了大鼠肝细胞色素４５０１Ａ１）和Ｖ７９（未表达Ｐ４５０１Ａ１）细胞生长的作用，目的为建立体外细胞毒模型。结果表明，在１．０、２．５、５．０μｍｏｌ·Ｌ１不同浓度下，ＢＰ７，８ｄｉｏｌ对ＸＥＭ２细胞产生很强的毒性，其毒性具有浓度和时间依赖性，在相同的浓度下ＢＰ７，８ｄｉｏｌ对Ｖ７９细胞并没有影响，同时也发现，细胞色素Ｐ４５０酶抑制剂αＮＦ对ＢＰ７，８ｄｉｏｌ引起的细胞毒具有保护作用。这些结果说明，ＢＰ７，８ｄｉｏｌ对ＸＥＭ２细胞毒性是通过细胞色素Ｐ４５０１Ａ１的作用引起的。
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| 关 键 词： | V79细胞株 XEM2细胞株 P4501A1 BP-7.8-diol 细胞毒性 |
Cytotoxicity of Benzo(a)pyrene 7,8-dihydrodiol on Genetically Engineered V79 Chinese Hamster Cells Expressing Rat Liver Cytochrome P450 1A1 |
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| Jing Rong Cui and N.P.E.Vermeulen . National Research Laboratory of Natural and Biomimetic Drugs,Beijing Medical University,Beijing.Cytotoxicity of Benzo(a)pyrene-7,8-dihydrodiol on Genetically Enginered V79 Chinese Hamster Cells Expresing Rat Liver Cytochrome P450 1A1[J].Journal of Chinese Pharmaceutical Sciences,1998(3). |
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| Authors: | Jing Rong Cui and NPEVermeulen National Research Laboratory of Natural and Biomimetic Drugs Beijing Medical University Beijing |
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| Institution: | Jing Rong Cui 1 and N.P.E.Vermeulen 2 1. National Research Laboratory of Natural and Biomimetic Drugs,Beijing Medical University,Beijing 100083 2. Division of Molecular Toxicology,Department of Pharmacochemistry,Faculty of Chemistry,Free Uni |
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| Abstract: | |
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| Keywords: | V79 cell line XEM2 cell line BP 7 8 diol Cytotoxicity P4501A1 |
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