| TRAIL受体在肿瘤细胞系上的表达及意义 |
| |
| 引用本文： | 韩丽辉,孙汶生,马春红,张利宁,曹英林,宋静.TRAIL受体在肿瘤细胞系上的表达及意义[J].细胞与分子免疫学杂志,2002,18(2):128-130. |
| |
| 作者姓名： | 韩丽辉 孙汶生 马春红 张利宁 曹英林 宋静 |
| |
| 作者单位： | 山东大学医学院微生物与免疫学教研室,山东,济南,250012 |
| |
| 基金项目： | 山东大学重点重大项目启动基金资助，No．413028 |
| |
| 摘 要： | 目的 检测TNF相关凋亡诱导配体（TRAIL）的受体，在来源于血液系统、肝脏、肺脏和大肠的8个肿瘤细胞系中的表达，并探讨其意义。方法 采用半定量RT－PCR，对TRAIL受体的表达进行半定量检测。结果 TRAIL凋亡通路中，能够诱导凋亡反应的死亡受体DR4和DR5，在所检测的肿瘤细胞系中都有表达，其中DR5在所有肿瘤细胞系中的表达水平均显著高于DR4（P＜0．05）。而能够竞争性与TRAIL诱导的凋亡反应的诱骗受体DcR1和DcR2，在所有的肿瘤细胞中都呈低水平表达或不表达。结论 DR5可能在TRAIL诱导凋亡的通路中发挥最重要的作用。TRAIL死亡受体和诱骗受体在肿瘤细胞系中的表达具有差异性，这种差异性可在一定程度上解释不同细胞对TRAIL诱导凋亡的敏感度。
|
| 关 键 词： | 肿瘤 RT－PCR TRAIL TRAIL受体 |
| 文章编号： | 1007-8738(2002)02-128-03 |
Expression and significance of TRAIL receptors in the tumor cell lines |
| |
| HAN Li-hui,SUN Wen-sheng,MA Chun-hong,ZHANG Li-ning,CAO Ying-lin,SONG Jing.Expression and significance of TRAIL receptors in the tumor cell lines[J].Journal of Cellular and Molecular Immunology,2002,18(2):128-130. |
| |
| Authors: | HAN Li-hui SUN Wen-sheng MA Chun-hong ZHANG Li-ning CAO Ying-lin SONG Jing |
| |
| Institution: | HAN Li-hui,SUN Wen-sheng,MA Chun-hong,ZHANG Li-ning,CAO Ying-lin,SONG Jing Department of Microbiology and Immunology,Shandong Medical University,Jinan 250012,Shandong Province,China |
| |
| Abstract: | AimTo detect the expressions of TRAIL receptors on 8 carcinoma cell lines of different origins and explore their significance. Methods The expressions of TRAIL receptors were assayed by semiquantitive RT-PCR.Results Both of the death receptor, DR4 and DR5 ,was detectable in all of the cell lines and the expression of DR5 was Signifiantly higher than that of DR4(P<0.05).The expressions of two decoy receptors (DcR1 and DcR2) were low or undetectable.ConclusionDR5 may play a key role in the apoptotic reaction induced by TRAIL.The expression level of TRAIL receptors on carcinoma cell lines are various,which may explain the difference in their sensitivity to TRAIL to a certain extent. |
| |
| Keywords: | carcinoma RT-PCR TRAIL receptor |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
