c-erbB-2反义寡核苷酸对人卵巢癌SKOV_3细胞增殖的抑制作用

目的探讨c-erbB-2基因反义寡核苷酸转染对人卵巢癌SKOV3细胞增殖的抑制作用及其分子机制。方法实验分空白对照组、正常对照组、c-erbB-2正义对照组及c-erbB-2反义治疗组。脂质体转染后不同时间分别进行MTT试验、集落形成试验、荧光显微镜检测、蛋白质荧光强度测定,以观察不同条件处理后各组细胞的增殖、凋亡、蛋白质表达有无差别。结果反义治疗组与正义对照组比较:转染72hOD值分别为0.201与1.298(P<0.01);克隆形成率分别为26.5%与76.5%(P<0.05);凋亡率分别为21.3%与7.5%(P<0.05),同时明显地下调c-erbB-2蛋白质的表达水平(P<0.05)。结论在卵巢癌的基因治疗中,c-erbB-2反义寡核苷酸能明显抑制人卵巢癌SKOV3细胞的增殖。

Inhibitory Effects of c-erbB-2 Antisense Oligodeoxynucleotides Transfection on the Human Ovarian Carcinoma SKOV3 Cell Lines

Objective To investigate the proliferating inhibition and the mechanisms of c-erbB-2 antisense oligodeoxynucleotides(ASODN) transfection on the human ovarian carcinoma SKOV_ 3 cell lines.Methods There were 4 groups in our study, blank control group,normal control group,c-erbB-2 sense control group and c-erbB-2 antisense experimental group.In the different time after liposome-mediated transfection,the cell proliferation,apoptosis,protein expressing level were observed by MTT assay,flow cytometry,fluorescent microscope and cloning test.Results According to the c-erbB-2 antisense experimental group and the c-erbB-2 sense control group,the OD-value were 0.201 and 1.298 (P< 0.01 ) respectively 72h after transfected;the cloning efficiency were 26.5% and 76.5% (P< 0.05 )respectively;the apoptosis rate were 21.3% and 7.5% (P< 0.05 )respectively;and the level of P185 protein was decreased statistically in ASODN group,respectively.Conclusion The study suggested that the proliferation of the human ovarian carcinoma SKOV_ 3 cell lines can be inhibited by the c-erbB-2 ASODN.