针灸对气虚大鼠红细胞免疫功能影响的实验研究

目的 :探讨针灸对红细胞免疫功能水平的影响。方法 :将 3 0只健康大鼠随机分为空白对照组、模型组和针灸组。针灸组每日行针刺及艾灸治疗 ,3 0min后 ,按游泳法复制大鼠气虚模型 ,连续重复 1 0天 ,次日各组采血抗凝 ,分别测定血液流变学指标、红细胞c3b受体花环率 (RBC .c3bRR)、红细胞免疫复合物花环率 (RBC .ICRR)。结果 :针灸组大鼠血液流变学指标均明显低于模型组 ,组间比较具有显著性差异 (P <0 0 1 ) ;模型组和针灸组的RBC .c3bRR均明显低于空白对照组 ,而RBC .ICRR明显高于空白对照组 (P <0 0 1 ) ;针灸组的RBC .c3bRR明显高于模型组 ,而RBC .ICRR明显低于模型组 (P <0 0 1 )。结论 :针灸对气虚大鼠的红细胞免疫功能低下具有一定的恢复作用

Experimental Study on the Effect of Acu-moxibustion on Red Blood Cell Immunologic Function in Rats with Qi-deficiency

Objective: To study the effect of acu-moxibustion on red blood cell immunologic function in rats with Qi-deficiency. Methods:30 Wistar rats were randomly and evenly divided into control group, model group and acu-moxibustion group. Qi-deficiency model was established by forcing the rat to swim continuously till it tended to sink spontaneously every day, ten days altogether. For rats of acu-moxibustion group, "Guanyuan"(CV 4), "Qihai"(CV 6) and bilateral "Zusanli"(ST 36) were punctured and stimulated electrically for 15 min, followed by performing scar-producing moxibustion for about 10 min. Whole blood viscosity (ηb) ratio, plasma viscosity (ηp) ratio, whole blood reduction viscosity ratio (RVR) and hematocrit (HCT) and erythrocyte electrophoresis time (EET), red blood cell c 3b receptor rosette rate (RBC.c 3bRR) and RBC immuno-complex rosette rate (RBC.ICRR) were detected and calculated before and after treatment respectively. Results: In comparison with control group, ηb and ηp ratios, RVR, HCT and EET of model group and acu-moxibustion increased significantly (P<0.05～0.01). Compared with model group, values of these 5 indexes in acu-moxibustion group lowered significantly (P<0.01). It indicates that acu-moxibustion can significantly improve blood rheology of Qi-deficiency rats. Compared with control group, RBC.ICRR values of model group and acu-moxibustion group increased significantly (P<0.01), while RBC.c 3bRR of the later two groups reduced evidently (P<0.01). Compared with model group, RBC.ICRR of acu-moxibustion group decreased evidently (P<0.01), while RBC.c 3bRR increased significantly (P<0.01), suggesting improvement of the cellular immunofunction.Conclusion: Acu-moxibustion can promote the restoration of the lowered red blood cell immunofunction in rats with Qi-deficiency.