硫酸酯化及羧甲基化虎奶多糖的抗病毒作用

目的 研究硫酸酯化及羧甲基化虎奶多糖的抗病毒作用效果及作用机理，探讨虎奶多糖应用于因柯萨奇病毒（ＣＶＢ３）导致克山病疾病预防的可能性。方法 采同变抑制法，在增养的Ｈｅｌａ细胞上测定硫酸酯化和羧甲基化虎奶多糖对ＨＳＶ－２和ＣＶＢ３２种病毒的抑制作用；用ＥＬＩＳＡ法测定这些多糖对ＨＢｓＡｇ的破坏作用。结果 ２种不同取代度的羧甲基化虎奶多糖及３种不同取代度的硫酸酯化虎奶多糖在浓度分别为０．５、０．２５、

Study on the antiviral activities of carboxymethylated and sulfonated Hunai polysaccharide

Objective To study the antivirus activity and the mechanism of carboxymethylated and sulfonated Hunai polysaccharide.Methods The inhibitory activities of the carboxymethylated and sulfonated Hunai polysaccharides against CVB_3,HSV-2 and HBsAg were evaluated in cell culture systems or with ELISA,respectively.Results The carboxymethylated and sulfonated Hunai polysaccharides (both with different degrees of substitution,DS) could inhibit HSV-2 virus in cell culture in the concentrations of 0.5,0.25 and 0.125 mg/ml.There was little difference in their inhibitory effect among the polysaccharides with different DS;but all of them exhibited no inhibition against CVB_3 or HBsAg.Conclusions All the polysaccharides exhibited strong inhibitory activity against HSV-2 in cell culture,but they have no inhibitory effect against CVB_3 in cell culture or HBsAg by ELISA.The results suggest that polysaccharides may inhibit the growth of virus by destroying their envelopes.