雷帕霉素与顺铂对Hep-2细胞的联合作用

目的 探讨联合应用顺铂和mTOR抑制剂雷帕霉素对喉癌Hep-2细胞的作用.方法 采用四甲基偶氮唑蓝比色法检测雷帕霉素和顺铂单独或联合应用对喉癌Hep-2细胞的抑制率,计算IC50值,探讨联合用药的效果;采用流式细胞术和Hoeehst33258免疫荧光检测药物单独或联合使用对喉癌Hep-2细胞凋亡的影响.结果 雷帕霉素的IC50值为11.03nmol/L,顺铂的IC50值为8.81 μmol/L.雷帕霉素可以拮抗Hep-2细胞增殖,使细胞停滞在G1期.联合使用雷帕霉素和顺铂进行化疗的协同治疗指数q>1.15(金氏公式),显示具有协同治疗作用;流式细胞术和免疫荧光检测显示联合使用雷帕霉素和顺铂可以明显增强对Hep-2细胞的凋亡水平.结论 联合应用雷帕霉素和顺铂可以明显提高对喉癌Hep-2细胞的杀伤效果,二者具有明显的协同作用.

Effect of chemotherapy with cisplatin and rapamycin against Hep-2 cells in vitro

Objective To evaluate the effect of combined use of rapamycin and cisplatin in the chemotherapy of Hep-2 cells in vitro.Methods The inhibitory effects of rapamycin and cisplatin, used alone or in combination, on the proliferation of Hep-2 cells were measured with MTT assay and median-effect plot analysis.The cell cycle changes after the treatment were analyzed using flow cytometry and Hoechst 33258 immunofluorescence staining.Results The IC50 of rapamycin and cisplatin for inducing growth arrest of Hep-2 ce...