Effect of human plasmalipoproteins on erythropoietic progenitor cells in serum-free cultures |
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Authors: | G. Konwalinka Ch. Breier Ch. Peschel D. Geissler H.-J. Lisch H. Braunsteiner |
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Affiliation: | (1) Department of Internal Medicine, University of Innsbruck, Anichstr. 35, A-6020 Innsbruck, Austria |
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Abstract: | Summary The purpose of the present study was to investigate the influence of human lipoproteins on CFU-e and BFU-e proliferation from human bone marrow in a serum-free system. In our previously described miniaturized agar system the main lipoprotein-density-classes from human plasma, namely very low density lipoproteins (VLDL), intermediate density lipoproteins (IDL), low density lipoproteins (LDL), high density lipoproteins2 (HDL2) and HDL3 and a mixture of all the five lipoproteins were added in rising concentrations (from 1/10 to normal human plasma concentration) to serum-free medium containing delipidated and deionized bovine serum albumin (BSA), iron saturated transferrin and erythropoietin. The results demonstrate that all lipoproteins markedly increased the CFU-e and BFU-e proliferation after 7 and 14 days of incubation, respectively. Moreover, the lipoproteins induced a shift towards a lower threshold concentration of erythropoietin. Serumlike conditions were obtained if LDL and the mixture of lipoproteins were added to serum-free medium. Furthermore, in the serum-free cultures a maturation to the mature erythrocyte could be found. |
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Keywords: | Human bone marrow cells Erythropoiesis Serum-free medium CFU-e BFU-e |
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