大鼠肝缺血及再灌注所致小肠过氧化损伤及丹参的保护作用

目的 观察大鼠肝缺血再灌注小肠过氧化损伤及丹参预处理的保护作用.方法 首先将SD大鼠随机分为正常对照组(CO组)、假手术组(SO组)、缺血再灌注组(IR组)、丹参预处理组(SM组),分别在肝缺血30、45、60 min时取上段空肠进行大体病理学检测;然后在肝缺血45 min条件下,动物亦随机分为4组(CO组、SO组、IR组、SM组),按再灌注后不同时间(0、3、12、24、72 h)分为5个亚组,每组5只.SM组在阻断第一肝门30 min前经尾静脉推注丹参注射液6 g/kg加生理盐水40 ml/kg,其余各组按40 ml/kg给予生理盐水尾静脉注入,SO组开腹后仅解剖肝门,不钳夹肝蒂.分别在再灌注0、3、12、24、72 h取上段空肠行病理学检查、丙二醛(MDA)含量测定、髓过氧化物酶(MPO)活性测定.结果 空肠黏膜损伤评分随肝缺血时限延长而加重;在肝缺血45 min再灌注不同时限点SM组空肠黏膜损伤较IR组明显减轻,且肠组织MDA含量、MPO活性均低于IR组(P<0.05).结论 肝缺血再灌注所致小肠明显淤血性损伤,MDA含量、MPO活性升高,丹参预处理对肝缺血再灌注所致小肠损伤具有保护作用.

Intestinal peroxide injury induced by ischemia/reperfusion and Salvia Miltiorrhiza pretreatment

Objective To study the intestinal peroxide injury induced by ischemia/reperfusion (I/R) and Salvia mihiorrhiza pretreatment. Methods SD rats were randomly divided into 4 groups: normal control group (CO), sham-operated group (SO), I/R group (IR), salvia miltiorrhiza pretreatment group (SM). Upper jejunal samples were taken at 30, 40, and 60 min of hepatic ischemia for general pathological examination. Under the condition of hepatic ischemia for 45 min, SD rats were also randomly divided into 4 groups : CO group, SO group, IR group, SM group, and each group were subdivided into 5 subgroups according to different reperfusion time (0, 3, 12, 24, 72 h), 5 in each subgroup. The rats in SM group were injected with 40 ml/kg saline and 6 g/kg Salvia Mihiorrhiza via caudal vein 30 min before clamping hepatic pedicle, and the rest groups were injected with 40 ml/kg normal saline via caudal vein. In SO group, only porta hepatic was dissected and hepatic pedicle was not clamped after laparotomy. The changes of the upper jejunum at different reperfusion time points (0, 3, 12, 24, 72 h) were observed under a light microscope. The content of malondialdehyde (MDA), and activity of myelopemxidase (MPO) were determined. Results Jejunal mucosal injury score was increased with the ischemia time. The score of jejunal mucosal injury in SM group was less than that of IR group, and the content of MDA and the activity of MPO were lower than in IR group (P<0.05) at different reperfusion time limits after hepatic ischemia for 45 min. Conclusion There is significant congestive injury at intestine in hepatic I/R injury, with an increased MDA content and MPO activity. Salvia miltiorrhiza pretreatment can protect he-patic I/R-induced intestinal injury.