丙烯酰胺染毒后胫神经及腓肠神经电生理指标的变化

目的观察丙烯酰胺亚慢性染毒对大鼠胫神经及腓肠神经电生理指标的影响。方法 36只健康雄性Wistar大鼠随机分为0、2、4、6、8、10周6组,以0周为对照组。染毒组给予丙烯酰胺(生理盐水溶液)40 mg/kg腹腔注射,每周3次。对照组同样方式注射生理盐水。分别于第0、2、4、6、10周测定胫神经运动神经传导速度(MNCV)和腓肠神经感觉神经传导速度(SNCV)。结果实验第4周时,染毒大鼠的MNCV减慢,波幅(AMP)降低(P<0.05);实验第6周开始,染毒组大鼠的潜伏期(LAT)延长(P<0.05),且各电生理指标持续改变至第10周。实验第4周时,染毒大鼠的SNCV减慢,潜伏期延长(P<0.05),且各电生理指标持续改变至第10周;其中,波幅改变无统计学意义。结论丙烯酰胺可致染毒大鼠胫神经及腓肠神经各电生理指标改变,并随染毒时间增加进行性加剧。

Changes of electrophysiological indices of tibial-and sural-nerve in rat after acrylamide exposure

Objective To explore the effect of subchronic acrylamide exposure on tibial-and sural-nerve electrophysiological function.Methods Thirty-six male Wistar rats were randomly divided into six groups.Rats were intraperitoneally administered with 40 mg/kg acrylamide 3 times per week for 2,4,6,8 and 10 weeks,respectively;the control rats were intraperitoneally treated with saline in the same way.The motor nerve conduction velocity(MNCV),latency(LAT)and amplitude(AMP)of tibial-nerve and sense nerve conduction velocity(SNCV),LAT and AMP of sural-nerve were respectively measured at 0,2,4,6,8,10 weeks of exposure.Results Compared with those in control,the MNCV and AMP of tibial-nerve in rats exposed to acrylamide for 4 weeks were significantly decreased(P<0.05),and the LAT were significantly lengthened from 6 weeks to 10 weeks exposed groups(P<0.05);while the SNCV of sural-nerve in rats of 4 weeks exposed group was also decreased significantly(P<0.05),besides LAT lengthened(P<0.05).Conclusion The results suggested that the exposure to acrylamide could impair the electrophysiological function of tibial motor nerve and the sural sense nerve.