CyclinD1及Survivin蛋白表达与鼻咽癌放疗敏感性的关系

目的研究CyclinDl和Survivin蛋白表达与鼻咽癌（nasopharyngealcarcinoma，NPC）放疗敏感性的关系。方法选取72例NPC患者放疗前活检组织标本（其中放疗敏感组49例，放疗不敏感组23例）．常规HE染色确定组织分型；免疫组化技术S-P法检测CyclinD1和Survivin蛋白表达情况；应用统计学软件进行统计学分析。结果72例NPC患者标本中，30例CyclinD1蛋白高表达，阳性表达率为41．7％。在NPC放疗敏感组和放疗不敏感组的高表达率分别为28．6％（14／49）和69．6％（16／23），两组比较差异有统计学意义（P〈0．05）；72例NPC患者标本中，34例Survivin蛋白高表达，阳性率为4712％，在放疗敏感组和放疗不敏感组高表达率分别为34．7％（17／49）和73．9％（17／23），两组比较差异有统计学意义（P〈0．05）。CyclinD1和Survivin蛋白的表达在NPC患者中呈正相关性，r=0．353（P〈0．05）。结论CyclinD1和Survivin蛋白的表达与NPC放射敏感性呈负相关。CyclinD1和Survivin可以作为预测NPC患者放疗敏感性的分子标志物。

Correlation between expression of Cyclin D1 protein and Survivin protein and radiosensitivity of na- sopharyngeal carcinoma

Objective To investigate the correlation between protein expression of Cyclin D1 and Survivin and radiosensitivity of nasopharyngeal carcinoma （NPC）. Methods 72 cases of NPC tissue speci- mens ,including 49 cases of radio-sensitive specimens and 23 cases of radio-resistant specimens, which were obtained before radiotherapy. The expression of Cyclin D1 protein and Survivin protein were detected by im- munohistochemical staining（S-P） method, and the results were analyzed. Results There were 30 cases were Cyclin D1 protein positive in the 72 cases, and the positive rate was 41.7%.The high expression rate of Cyclin D1 in radio-sensitive group and radio-resistant group were 28.6% （14/49） and 69.6% （16/23）, and the dif- ference had statistical significance （P〈 0.05 ）; There were 34 cases were Survivin protein positive in the 72 cases, and the positive rate was 47.2%. The high expression rate of Survivin in radio-sensitive group and ra- dio-resistant group were 34.7% （17/49）and 73.9% （17/23）, and the difference had statistical significance （P〈 0.05）. The expression of Cyclin D1 had positive correlation with the expression of Survivin in NPC pa- tients, r=0.353 （P〈 0.05）. Conclusion The expression of Cyclin D1 and Survivin had negative correlation with radiosensitivitv of NPC, which could be a useful predictor of radiosensitivity in NPC.