甘露消毒丹对柯萨奇病毒体外抑制作用的实验研究

目的：观察甘露消毒丹全方、残方及加味方对柯萨奇病毒的体外抑制作用。方法：采用组织培养技术，在培养细胞中观察甘露消毒丹全方、残方及加味方水煎液分别对培养细胞的最高无毒浓度、对柯萨奇病毒增殖产量的影响以及在不同时间内抑毒效果。结果：甘露消毒丹全方、残方及加味方对细胞的最高无毒浓度均为１：４０（即２５ｇ／Ｌ），该浓度对柯萨奇病毒Ｂ２、Ｂ３、Ｂ４株在培养细胞内的增殖有明显的抑制作用，抑制指数均〉２；全方对

Experimental Study on Inhibitory Effect of Ganlu Xiaodu Dan on Coxackie Virus in Vitro

OBJECTIVE: To observe the Coxackie virus inhibitory effect of Ganlu Xiaodu Dan (GLXDD), including complete recipe, incomplete recipe and supplemented recipe of it. METHODS: Using tissue culture technique, the maximal non-toxic concentration of drug on cultured cells, the influence of drug on proliferation of virus in cultured cells and the virus inhibitory effect in different time of action were observed. RESULTS: The maximal non-toxic concentration of GLXDD was 1:40 (25 g/L), no matter whether the recipe was complete, incomplete or supplementary. CLXDD in that concentration could inhibit significantly the proliferation of Coxackie virus B2, B3 and B4 strains, the inhibition index being over 2 and the output of these strains treated with complete recipe of GLXDD was lower significantly than that of the control group (P < 0.05). CONCLUSION: GLXDD, including its complete recipe, incomplete recipe and supplementary recipe, could inhibit the replication of Coxackie virus in cultured cells.