谷胱甘肽对顺铂所致肾小管上皮细胞毒性的保护作用

目的 探讨谷胱甘肽（GSH）对顺铂所致不同性别大鼠肾小管上皮细胞毒性的影响。方法 从不同性别的大鼠分离的肾小管上皮细胞接种于96孔培养液，培养24h后加入一系列浓度的顺铂，或在加入顺铂前的16和4h，分别加入GSH合成抑制剂BSO和GSH合成前体物半胱氨酸，再培养24h后用MTT方法检测细胞存活率。结果：顺铂对雌雄大鼠肾小管上皮细胞具有形状相似的剂量－反应关系曲线，半数抑制浓度（IC50）分别为0.178mmol/L和0.182mmol/L；BSO能使2组IC50均2降低为0.001mmol/L，可使剂量－反应曲线左移，而半胱氨酸则可使2组IC50均升高，均大于5mmol/L，使剂量－反应曲线下沉。结论 顺铂对雌雄大鼠肾小管上皮细胞同样具有明显的毒性；BSO和半胱氨酸可分别增强和降低顺铂的毒性，间接证明细胞内GSH对顺铂所致大鼠肾小管上皮细胞毒性有保护作用，且与性别无前。

Protective effects of glutathione on cisplatin-induced toxicity to primary cultured rat renal tubular epithelial cells without sex-dependent difference

To investigate the protective effects of glutathione (GSH) on cisplatin-induced toxicity to renal tubular epithelial cells isolated from different sex rats. Renal tubular epithelial cells were isolated from different sex rats and inoculated into 96-well culture plates, after 24 hours' preincubation, a series of concentration of cisplatin, in the presence and absence of DL-buthionine-(S,R)-sulfoximine(BSO), inhibitor of GSH synthesis, or L-cysteine, precursor of GSH synthesis, were added, and after 24 hours' coincubation, MTT assay was performed to test cell viability. The concentrations of cisplatin that inhibited 50% cell growth(IC 50 ) of renal tubular epithelial cells from female or male rats were 0.182 mmol/L and 0.178 mmol/L, respectively, and they had similar dose-response curves; BSO made both of IC 50 of cisplatin lower down to 0.001 mmol/L, while L-cysteine made both of IC 50 of cisplatin increase up to more than 5 mmol/L. [Conclusion] Cisplatin is dramatically toxic to renal tubular epithelial cells from female or male rats; BSO can enhance cisplatin-induced cytotoxicity, while L-cysteine can protect cisplatin-induced cytotoxicity, it is indirectly proved that GSH can protect cisplatin-induced toxicity to renal tubular epithelial cells from rats without sex-dependent difference.