胰蛋白酶消化法对弓形体速殖子的影响

用两次胰蛋白酶消化,共10次离心洗涤的方法,自感染弓形体小鼠的腹水内提纯弓形体速殖子。经台盼蓝染色、间接荧光抗体法检测与电镜观察,检测虫体活力、抗原性及形态变化。试验前,弓形体的台盼蓝自然着色(死亡)率是6％。末次离心洗涤后,胰蛋白酶消化组的弓形体有65％被台盼蓝染色;水洗对照组为41％。两组有显著性差异(P< 0.01)。用弓形体阳性人血清(滴度1:16),检测两组弓形体,与正常对照虫体相比,其荧光着色特点与滴度均未见明显改变。透射电镜发现,胰蛋白酶消化组与水洗对照组的弓形体,大多数的质膜呈波浪形并有一些疣状突,尤其是胰蛋白酶消化的虫体,其类锥体端的质膜肿胀、增厚,层次消失。

Effect of trypsinase digestion on tachyzoites of Toxoplasma gondii

The peritoneal fluid of mica infected with Toxoplasma gondii was treated twice with double trypsinase digestion and normal Saline centrifugal washing for ten times to purify tachyzoites.Before treatment,6% of the tachyzpites-of Toxoplasma gondii were naturally stained(dead ).But following the final washing,65% of the parasites were stained in group of trypsinase digestion,41% of that in the control group The difference is highly significant (P<0.01).However,no change was found in feature and titre of IFA of the two groups.During the testing,human serum of anti-Toxoplasma gondii with titre 1:16 was used with normal parasite as control.Electron microscopic studies show that the plasmolemma of most tachyzoites treated by trypsinase or normal saline become wavy and some warty projections appear.