脑卒中急性期血清白介素-10和外周血T淋巴细胞Ag-NORs的动态变化

目的 研究脑卒中急性期血清IL-10的动态变化规律以及细胞免疫功能变化.方法 选择3 d内首次发病患者,IL-10采用ELISA方法于发病72 h内、第6～8天及第14～15天抽血检测,Ag-NORs于发病72 h内、第14～15天抽血,对外周血T淋巴细胞染色分析,以核仁银染面积与细胞核面积比值（I.S%）作为Ag-NORs检测指标,对照组抽血检测1次.结果 脑卒中组血清IL-10水平各时间点均较对照组明显增高（P＜0.05）,不同时间无明显变化（P＞0.05）,不同病情亦无明显差异（P＞0.05）.脑卒中患者急性期Ag-NORs水平（I.S%）明显降低（P＜0.01）,发病后第14～15 d较72 h内高（P＜0.01）,但仍低于对照组（P＜0.01）.脑梗死组同一时期重度患者Ag-NORs水平较轻度患者低（P＜0.05）.结论 IL-10在脑卒中急性期明显增高,参与了卒中炎性损伤的病理过程,有望成为治疗脑卒中的潜在药物.且脑卒中急性期细胞免疫功能低下.

Changes of serum IL-10 and Ag-NORs of T lymphocytes in patients with acute cerebral stroke

Objective To study the changes of srrum IL-10 and Ag-NORs of T lymphocytes in patients with acute cerebral stroke. Methods Patients with first-ever acute stroke within the first 3 days after stroke onset were included. The level of serum IL-10 was measured using enzyme linked immunosorbent assay(ELISA). The ratio of nuclear area by silver staining to nuclear area(I.S%) was as the index of Ag-NORs. Results 1)Compared with those of the control, the level of serum IL-10 was increased in the infarction group and cerebral hemorrhage group(P<0.05) at all stages. The level of serum IL-10 was not obviously different in patients with different stages (P>0.05). 2) Compared with those of control, the level of Ag-NORs of T lymphocytes in patients with acute cerebral stroke was decreased(P<0.01); the level of Ag-NORs in the second week was higher than that in 72 hours(P<0.01), but lower than that in control group(P<0.01). The level of Ag-NORs of T lymphocytes in patients with serious symptoms was lower (P<0.05). Conclusions 1) The level of anti-inflammatory cytokine IL-10 was greatly increased in patients with acute cerebral stroke, it might be involved in the inflammatory process of stroke. IL-10 may become a potential therapeutic drug. 2) The cell immunological function in patients with acute cerebral stroke decreased, and may be associated with the pathogenetic process of ischemic stroke.