COX-2和Bax在非小细胞肺癌中的表达及意义

目的探讨非小细胞肺癌（NSCLC）中环氧化酶-2（COX-2）和Bax的表达及其临床意义。方法采用免疫组织化学方法检测57例NSCLC癌组织（腺癌/鳞癌）、相应配对的15例正常组织及57例淋巴结COX-2蛋白和Bax蛋白的表达水平,计算其阳性百分比进行相关性分析。结果NSCLC组织中COX-2蛋白表达高于癌旁正常组织（P〈0.05）,腺癌高于鳞癌（P〈0.05）,与肿瘤组织的病理分级相关,且与淋巴结转移呈正相关（P〈0.05）,但与患者的年龄、性别无明显相关（P〉0.05）;Bax蛋白表达低于癌旁正常组织（P〈0.05）,腺癌高于鳞癌（P〈0.05）,且与淋巴结转移呈负相关（P〈0.05）,与年龄、性别、病理分级间差异无统计学意义（P〉0.05）。结论COX-2在NSCLC尤其是肺腺癌中表达上调,Bax蛋白在NSCLC组织中表达过低,COX-2可能通过降低Bax表达,抑制细胞凋亡参与NSCLC的发生、发展。

Expression and significance of cyclooxygenase-2 and apoptosis inhibitor-Bax in Non-small cell lung cancer

Objective To investigate the expression of cyclooxygenase-2(COX-2) and Bax protein in non-small cell lung cancer(NSCLC),and its clinical significance and correlations of COX-2 and Bax.Methods Samples were obtained from 57 cases NSCLC tissues(including adenocarcinoma and squamous cell carcinoma),15 sampls nomal lung tissues and 57 samples Lymphoid tissue.The expression level of COX-2 protein and Bax protein in these samples were examined by immunohistochemistry.The relationship between COX-2 and Bax were analyzed by positive percentage.Results The expression of COX-2 protein in NSCLC tissues is higher than nomal tissue which were obained nearly(P<0.05),and the expression of COX-2 protein in the adenocarcinoma was higher than in the squamous cell carcinoma.That was positive related with divide degree of tumor tissues,and positive related with lymph node transfer(P<0.05),but was non-related with age and gender(P>0.05);The expression of Bax protein in NSCLC tissues was lower than nomal tissue which were obained neraly(P<0.05),and the expression of Bax protein in the adenocarcinoma was higher than in the squamous cell carcinoma(P<0.05),it was related with negative related with lymph node transfer(P<0.05),but was non-related with age,gender,pathologic ratings(P>0.05).there was relationship between COX-2 and Bax(P<0.05).Conclusions The expression of COX-2 protein is up-regulated in NSCLC tissues,particularly in adenocarcinoma.The expression of Bax proiein is over-low in NSCLC tissues.COX-2 may participate in development and progression of NSCLC by lower expression of Bax inducing cell apoptosis.