| P-170糖蛋白在多药耐药(MDR)K562/ADM细胞中的定位研究 |
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| 引用本文： | 高船舟,曲淑贤,吕广艳,郝立宏,杨佩满. P-170糖蛋白在多药耐药(MDR)K562/ADM细胞中的定位研究[J]. 大连医科大学学报, 2002, 24(2): 91-93 |
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| 作者姓名： | 高船舟 曲淑贤 吕广艳 郝立宏 杨佩满 |
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| 作者单位： | 1. 大连医科大学分析测试中心,辽宁,大连,116027
2. 大连医科大学组织胚胎学教研室,辽宁,大连,116027 |
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| 摘 要： | 目的：确定P-170糖蛋白在多药耐药（MDR）K562/ADM细胞中表达的位置，方法：通过免疫反应并利用流式细胞仪（FCM）检测表达P-170糖蛋白细胞的百分含量；用免疫电镜技术检测P-170糖蛋白在细胞中的位置及其表达水平。结果：敏感株K562细胞与耐药株K562/ADM细胞对P-170糖蛋白的表达有非常明显的差异；不同的K562/ADM细胞表达P-170糖蛋白的量差别较大；少量的K562细胞也有极少的P-170糖蛋白的表达，细胞表达的P-170糖蛋白位于细胞膜上，结论：多药耐药K562/ADM细胞表达的P-170糖蛋白最终被镶嵌在细胞膜上；在该蛋白质从被表达，修饰和转运到细胞膜上的过程中，该蛋白质不具有成熟P-170糖蛋白的特异构象。
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| 关 键 词： | 多药耐药 P－170糖蛋白 免疫电镜 K562／ADM细胞 |
| 文章编号： | 1000-5676(2002)02-0091-03 |
| 修稿时间： | 2002-03-11 |
Confirming the position of P-glycoprotein in K562/ADM cells by the method of immune electron microscope |
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| GAO Chuan-zhou,QU Shu-xian,L Guang-yan,HAO Li-hong. Confirming the position of P-glycoprotein in K562/ADM cells by the method of immune electron microscope[J]. Journal of Dalian Medical University, 2002, 24(2): 91-93 |
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| Authors: | GAO Chuan-zhou QU Shu-xian L Guang-yan HAO Li-hong |
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| Affiliation: | Central of Analysis and Test, Dalian Medical University, Dalian 116027, China;Central of Analysis and Test, Dalian Medical University, Dalian 116027, China;Central of Analysis and Test, Dalian Medical University, Dalian 116027, China;Central of Analysis and Test, Dalian Medical University, Dalian 116027, China;Central of Analysis and Test, Dalian Medical University, Dalian 116027, China |
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| Abstract: | Objective: To confirm the position of P-glycoprotein in K562/ADM cells with immune electron microscope(IEM) technology. Method: The percent of K562/ADM cells of expressing P-glycoprotein was mensurated by Flow cytometer (FCM); The position and expressing level of P-glycoprotein in K562/ADM cells were confirmed with IEM. Results: There are obvious differentiation of expressing level of P-glycoprotein in K562 and K562/ADM cells, K562/ADM cell line is higher than K562. Every cell of K562/ADM expresses P-glycoprotein with different quantity, but a few of K562 cells expressed a little P-glycoprotein. The P-glycoprotein is incrusted in cell membrane finally. Conclusions: P-glycoprotein is incrusted in cell membrane of K562 and K562/ADM cells. |
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| Keywords: | multidrug resistance (MDR) P-glycoprotein immune electronic microscope |
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