替普瑞酮对胃上皮细胞增殖、迁移及凋亡的影响

目的：观察替普瑞酮对人正常胃上皮细胞增殖、迁移及凋亡等生物学现象的影响。方法体外培养人正常胃上皮细胞，M T T法检测不同浓度替普瑞酮对胃上皮细胞的增殖作用并确定替普瑞酮最佳作用浓度。T ransw ell实验、划痕实验检测替普瑞酮最佳作用浓度对胃上皮细胞运动能力及迁移作用的影响。流式细胞法检测替普瑞酮最佳作用浓度对胃上皮细胞凋亡的影响。结果替普瑞酮作用于胃上皮细胞24 h后，开始出现促进胃上皮细胞增殖的现象，从10～80μmol／L随着浓度增加，促进作用越明显，而＞80～320μmol／L随着浓度增加，促进作用无明显改变，据此确定药物最佳作用浓度为80μmol／L。80μmol／L浓度替普瑞酮处理胃上皮细胞24 h后，T ransw ell实验显示，对照组的迁移率为1．328±0．208，加药组为3．338±0．293，小室膜下蓝染的细胞加药组明显高于对照组（P＜0．01）；划痕实验显示加药组划痕区域细胞迁移率1．00±0．18，对照组0．72±0．08，加药组迁移率明显高于对照组（P＜0．05）。80μmol／L浓度替普瑞酮处理胃上皮细胞48 h后，加药组细胞凋亡率（11．90±1．53）％低于对照组（25．61±0．15）％，差异有统计学意义（ P＜0．01）。结论替普瑞酮能够促进人胃上皮细胞增殖及迁移，抑制人胃上皮细胞凋亡。

Influences of teprenone on proliferation,migration and apoptosis of gastric epithelial cell line

Objective This study was to observe the biological effects of eprenone on proliferation ,migration and apoptosis in human gastric epithelial cell line .Methods Human gastric epithelial cells GES‐1 were cultured in vitro .MTT assay were used to e‐valuate the proliferation of GES‐1 cells in different concentrations of teprenone and ensure the appropriate drug concentration .T ran‐swell test and scratch test were used to detect the migration ability of GES‐1 cells treated with appropriate concentration of eprenone .Flow cytometry analysis were used to detect the apoptosis of GES‐1 cells treated by the appropriate concentration of eprenone .Results Treated with eprenone for 24 h ,the proliferation of GES‐1 cells were increased as the concentration of teprenone from 10 to 80 μmol/L ,but from 80 to 320 μmol/L ,the promoting effect showed no staticall significant changes .So the appropriate drug concentration was determined to be 80 μmol/L .Treated with teprenone (80 μmol/L) for 24 h ,the transwell test showed that the migration rate of the teprenone group was 3 .338 ± 0 .293 and the control group was 1 .328 ± 0 .208 .So the number of staining blue cells in eprenone group were more than in control group obviously under membrane of transwell chambers (P<0 .01) .Scratch test showed that the migration rate of the eprenone group was 1 .00 ± 0 .18 and the control group was 0 .72 ± 0 .08 .Similarly ,the migration rate of eprenone group was higher than the control group(P<0 .05) .Treated with teprenone (80 μmol/L) for 48 h ,the apoptosis rate of the teprenone group was (11 .90 ± 1 .53)% and the control group was (25 .61 ± 0 .15)% ,the cellular apoptosis of eprenone group was lower than the control group (P<0 .01) .Conclusion Teprenone can promote the proliferation and migration , inhibit the apoptosis of GES‐1 cells .